1887

Abstract

The report of four novel mammalian pathogenic species of the genus prompted us to study the use of biochemical assays to differentiate the Oomycota mammalian pathogens and spp. We investigated the reaction of 23 and eight species in various biochemical assays. Because the morphological features of the Oomycota species are similar to those of species in the Entomophthoramycota and Mucormycota, five fungal species with coenocytic hyphae were also included. We found that mammalian and plant isolates of spp. all hydrolysed sucrose, but species and the fungal strains did not. In addition, both spp. and spp. were found to be maltose-positive, whereas fungal strains did not hydrolyse this sugar. The fungal species and thermo-sensitive and were urease-negative, but the mammalian spp. and spp. hydrolysed urea within 24 h. These findings suggest these assays can be used for the presumptive differentiation of mammalian Oomycota species in the laboratory.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000111
2015-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/64/8/862.html?itemId=/content/journal/jmm/10.1099/jmm.0.000111&mimeType=html&fmt=ahah

References

  1. Brown T.A., Grooters A.M., Hosgood G.L. 2008; In vitro susceptibility of Pythium insidiosum and a Lagenidium sp. to itraconazole, posaconazole, voriconazole, terbinafine, caspofungin, and mefenoxam. Am J Vet Res 69:1463–1468 [View Article][PubMed]
    [Google Scholar]
  2. Calvano T.P., Blatz P.J., Vento T.J., Wickes B.L., Sutton D.A., Thompson E.H., White C.E., Renz E.M., Hospenthal D.R. 2011; Pythium aphanidermatum infection following combat trauma. J Clin Microbiol 49:3710–3713 [View Article][PubMed]
    [Google Scholar]
  3. De Cock A.W., Mendoza L., Padhye A.A., Ajello L., Kaufman L. 1987; Pythium insidiosum sp. nov., the etiologic agent of pythiosis. J Clin Microbiol 25:344–349[PubMed]
    [Google Scholar]
  4. Dunbar M.D., Wamsley H.L. 2009; What is your diagnosis? Lymph node cytology from a dog. Vet Clin Pathol 38:91–93 [View Article][PubMed]
    [Google Scholar]
  5. Gaastra W., Lipman L.J., De Cock A.W.A.M., Exel T.K., Pegge R.B., Scheurwater J., Vilela R., Mendoza L. 2010; Pythium insidiosum: an overview. Vet Microbiol 146:1–16 [View Article][PubMed]
    [Google Scholar]
  6. Grooters A.M. 2003; Pythiosis, lagenidiosis, and zygomycosis in small animals. Vet Clin North Am Small Anim Pract 33:695–720, v [View Article][PubMed]
    [Google Scholar]
  7. Grooters A.M., Hodgin E.C., Bauer R.W., Detrisac C.J., Znajda N.R., Thomas R.C. 2003; Clinicopathologic findings associated with Lagenidium sp. infection in 6 dogs: initial description of an emerging oomycosis. J Vet Intern Med 17:637–646 [View Article][PubMed]
    [Google Scholar]
  8. Hallmon C.F., Schreiber E.T., Vo T., Bloomquist A. 2000; Field trials of three concentrations of Laginex as biological larvicide compared to Vectobac-12AS as a biocontrol agent for Culex quinquefasciatus . J Am Mosq Control Assoc 16:5–8[PubMed]
    [Google Scholar]
  9. Hensel P., Greene C.E., Medleau L., Latimer K.S., Mendoza L. 2003; Immunotherapy for treatment of multicentric cutaneous pythiosis in a dog. J Am Vet Med Assoc 223:215–218, 197 [View Article][PubMed]
    [Google Scholar]
  10. Kwon-Chung K.J. 2012; Taxonomy of fungi causing mucormycosis and entomophthoramycosis (zygomycosis) and nomenclature of the disease: molecular mycologic perspectives. Clin Infect Dis 54:(Suppl. 1)S8–S15 [View Article][PubMed]
    [Google Scholar]
  11. Lass-Flörl C. 2009; Zygomycosis: conventional laboratory diagnosis. Clin Microbiol Infect 15:(Suppl. 1)60–65 [View Article][PubMed]
    [Google Scholar]
  12. Mendoza L., Newton J.C. 2005; Immunology and immunotherapy of the infections caused by Pythium insidiosum . Med Mycol 43:477–486 [View Article][PubMed]
    [Google Scholar]
  13. Mendoza L., Prendas J. 1988; A method to obtain rapid zoosporogenesis of Pythium insidiosum . Mycopathologia 104:59–62 [View Article][PubMed]
    [Google Scholar]
  14. Mendoza L., Vilela R. 2013; The mammalian pathogenic oomycetes. Curr Fungal Infect Rep 7:198–208 [View Article]
    [Google Scholar]
  15. Mendoza L., Prasla S.H., Ajello L. 2004; Orbital pythiosis: a non-fungal disease mimicking orbital mycotic infections, with a retrospective review of the literature. Mycoses 47:14–23 [View Article][PubMed]
    [Google Scholar]
  16. Reinprayoon U., Permpalung N., Kasetsuwan N., Plongla R., Mendoza L., Chindamporn A. 2013; Lagenidium sp. ocular infection mimicking ocular pythiosis. J Clin Microbiol 51:2778–2780 [View Article][PubMed]
    [Google Scholar]
  17. Ribes J.A., Vanover-Sams C.L., Baker D.J. 2000; Zygomycetes in human disease. Clin Microbiol Rev 13:236–301 [View Article][PubMed]
    [Google Scholar]
  18. Thianprasit M., Chaiprasert A., Imwidthaya P. 1996; Human pythiosis. Curr Top Med Mycol 7:43–54[PubMed]
    [Google Scholar]
  19. Thitithanyanont A., Mendoza L., Chuansumrit A., Pracharktam R., Laothamatas J., Sathapatayavongs B., Lolekha S., Ajello L. 1998; Use of an immunotherapeutic vaccine to treat a life-threatening human arteritic infection caused by Pythium insidiosum . Clin Infect Dis 27:1394–1400 [View Article][PubMed]
    [Google Scholar]
  20. Vilela R., Silva S.M.S., Riet-Correa F., Dominguez E., Mendoza L. 2010; Morphologic and phylogenetic characterization of Conidiobolus lamprauges recovered from infected sheep. J Clin Microbiol 48:427–432 [View Article][PubMed]
    [Google Scholar]
  21. Vilela R., Taylor J.W., Walker E.D., Mendoza L. 2015; Lagenidium giganteum pathogenicity in mammals. Emerg Infect Dis 21:290–297 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000111
Loading
/content/journal/jmm/10.1099/jmm.0.000111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error