1887

Abstract

Plasmid-mediated quinolone resistance (PMQR) refers to a family of closely related genes that confer decreased susceptibility to fluoroquinolones. PMQR genes are generally associated with integrons and/or plasmids that carry additional antimicrobial resistance genes active against a range of antimicrobials. In Ho Chi Minh City (HCMC), Vietnam, we have previously shown a high frequency of PMQR genes within commensal . However, there are limited available sequence data detailing the genetic context in which the PMQR genes reside, and a lack of understanding of how these genes spread across the . Here, we aimed to determine the genetic background facilitating the spread and maintenance of the dominant PMQR gene circulating in HCMC. We sequenced three -carrying plasmids in their entirety to understand the genetic context of these -embedded plasmids and also the association of -mediated quinolone resistance with other antimicrobial resistance phenotypes. Annotation of the three -containing plasmids revealed a -containing transposon with a closely related structure. We screened 112 -positive commensal isolated in the community and in a hospital in HCMC to detect the common transposon structure. We found the same transposon structure to be present in 71.4 % (45/63) of -positive hospital isolates and in 36.7 % (18/49) of -positive isolates from the community. The resulting sequence analysis of the environment suggested that genes are widely distributed and are mobilized on elements with a common genetic background. Our data add additional insight into mechanisms that facilitate resistance to multiple antimicrobials in Gram-negative bacteria in Vietnam.

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2015-08-01
2021-03-01
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