1887

Abstract

The gene was detected in 14 meticillin-susceptible isolates recovered from outpatients with community-onset infections in a county hospital in China. The MIC of linezolid was 4 μg ml in eight isolates and 2 μg ml in six isolates. All isolates were susceptible to vancomycin and teicoplanin, but had elevated MICs for penicillin (0.5–128 μg ml), chloramphenicol (2–32 μg ml), clindamycin (0.5–128 μg ml) and erythromycin (4–128 μg ml). Nine isolates had mutations on domain V of 23S rRNA and/or the ribosomal L proteins that were not located close to the linezolid-binding pocket. Southern blotting experiments demonstrated that the genes in all 14 isolates resided on plasmids. Sequence analysis of the 5.6 kb -carrying plasmid segment revealed 99 % identity to the corresponding sequences in plasmid pSS-01 from animal staphylococci and plasmid pRM-01 from human staphylococci. Five isolates belonged to sequence type (ST)188 and three to ST965; the two ST types were previously reported in isolates of animal origin in some areas of China. These results indicate that the -carrying plasmids in this study are likely of animal origin. The present study shows that -harbouring isolates have emerged in some areas of China and that -carrying isolates may be transmitted between animals and humans.

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2015-08-01
2020-01-23
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