1887

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Volume 64, Issue 7

Genital carriage of the genus in pregnancy: species distribution and antibiotic susceptibility Free

Abstract

Recent reports have hypothesized that colonization of the maternal genital tract with non-capsulated could result in neonatal invasive disease. In this study, genital carriage of the genus was investigated in 510 pregnant women attending an Italian hospital for routine controls. Overall, vaginal carriage of the genus was 9.0 % (46/510). A high colonization rate with (37/510, 7.3 %) was found; other species, such as (7/510, 1.4 %) and (2/510, 0.4 %), were detected for the first time in the genital flora by 16S rRNA gene sequencing. Notably, no was identified, in agreement with previous investigations indicating that this species is rarely isolated from the genito-urinary tract of pregnant women. No antibiotic resistance was detected in and , but quite a high degree of ampicillin (10/37, 27 %) and ciprofloxacin (3/37, 8.1 %) resistance was observed in . Five ampicillin-resistant isolates were β-lactamase producers, whereas five isolates exhibited a β-lactamase-negative ampicillin-resistant (BLNAR) phenotype. Sequencing of penicillin-binding protein 3 revealed that Val511Ala, Asn526Ser, Ala530Ser and Thr574Ala changes were associated with BLNAR phenotypes. Two ciprofloxacin-resistant isolates carried substitutions in both GyrA (Ser84Phe and Asp88Tyr) and ParC (Ser84Tyr and Met198Leu); the other ciprofloxacin-resistant isolate had substitutions in ParC, only (Ser138Thr and Met198Leu). In conclusion, ∼10 % of pregnant women carried a species of in their genital tract. The emergence of non-β-lactamase-mediated resistance in genital is a matter of concern because of the risk of mother-to-baby transmission.

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© 2015 The Authors
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2015-07-01
2024-04-20
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Genital carriage of the genus Haemophilus in pregnancy: species distribution and antibiotic susceptibility
J. Med. Microbiol. 64, 724 (2015); https://doi.org/10.1099/jmm.0.000083
/content/journal/jmm/10.1099/jmm.0.000083
/content/journal/jmm/10.1099/jmm.0.000083
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