1887

Abstract

Accurate susceptibility results on antibiotic-resistant bacteria are essential for proper treatment of infections. In this study, 100 metallo-β-lactamase (MBL)-producing strains and 95 isolates with carbapenemase (KPC) were tested for carbapenem susceptibility using two automated platforms, the Phoenix and Vitek-2 systems, and a manual Etest. Phoenix showed higher categorical agreements (97 % for imipenem and 94 % for meropenem) compared with those from Vitek-2 (92 and 74 %) and Etest (89 and 96 %), respectively, when testing MBL strains. Categorical agreement for imipenem tests with KPC-producing strains was 88.4 % with the Phoenix system, 83.2 % with the Vitek 2 system and 90.5 % with the Etest. Categorical agreement was 100 % for all tests with ertapenem. In conclusion, the Phoenix system demonstrated a higher accuracy than Vitek-2 in testing carbapenemase-producing strains, particularly in MBL strains.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000067
2015-06-01
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/64/6/620.html?itemId=/content/journal/jmm/10.1099/jmm.0.000067&mimeType=html&fmt=ahah

References

  1. Bulik C. C. , Fauntleroy K. A. , Jenkins S. G. , Abuali M. , LaBombardi V. J. , Nicolau D. P. , Kuti J. L. . ( 2010;). Comparison of meropenem MICs and susceptibilities for carbapenemase-producing Klebsiella pneumoniae isolates by various testing methods. . J Clin Microbiol 48:, 2402–2406, [CrossRef] [PubMed].
    [Google Scholar]
  2. Carmeli Y. , Akova M. , Cornaglia G. , Daikos G. L. , Garau J. , Harbarth S. , Rossolini G. M. , Souli M. , Giamarellou H. . ( 2010;). Controlling the spread of carbapenemase-producing Gram-negatives: therapeutic approach and infection control. . Clin Microbiol Infect 16:, 102–111.[CrossRef]
    [Google Scholar]
  3. CLSI ( 2012;). Performance Standards for Antimicrobial Susceptibility Testing, 22nd Informational Supplement M100-S22. Wayne, PA:: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  4. CLSI ( 2015;). Performance Standards for Antimicrobial Susceptibility Testing, 25th Informational Supplement M100-S25. Wayne, PA:: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  5. Doern C. D. , Dunne W. M. Jr , Burnham C. A. . ( 2011;). Detection of Klebsiella pneumoniae carbapenemase (KPC) production in non-Klebsiella pneumoniae Enterobacteriaceae isolates by use of the Phoenix, Vitek 2, and disk diffusion methods. . J Clin Microbiol 49:, 1143–1147, [CrossRef] [PubMed].
    [Google Scholar]
  6. Espedido B. A. , Thomas L. C. , Iredell J. R. . ( 2007;). Metallo-β-lactamase or extended-spectrum β-lactamase: a wolf in sheep's clothing. . J Clin Microbiol 45:, 2034–2036, [CrossRef] [PubMed].
    [Google Scholar]
  7. Giakkoupi P. , Tzouvelekis L. S. , Daikos G. L. , Miriagou V. , Petrikkos G. , Legakis N. J. , Vatopoulos A. C. . ( 2005;). Discrepancies and interpretation problems in susceptibility testing of VIM-1-producing Klebsiella pneumoniae isolates. . J Clin Microbiol 43:, 494–496, [CrossRef] [PubMed].
    [Google Scholar]
  8. Kollef K. E. , Schramm G. E. , Wills A. R. , Reichley R. M. , Micek S. T. , Kollef M. H. . ( 2008;). Predictors of 30-day mortality and hospital costs in patients with ventilator-associated pneumonia attributed to potentially antibiotic-resistant Gram-negative bacteria. . Chest 134:, 281–287, [CrossRef] [PubMed].
    [Google Scholar]
  9. Queenan A. M. , Bush K. . ( 2007;). Carbapenemases: the versatile β-lactamases. . Clin Microbiol Rev 20:, 440–458, [CrossRef] [PubMed].
    [Google Scholar]
  10. Sabuda D. M. , Laupland K. , Pitout J. , Dalton B. , Rabin H. , Louie T. , Conly J. . ( 2008;). Utilization of colistin for treatment of multidrug-resistant Pseudomonas aeruginosa . . Can J Infect Dis Med Microbiol 19:, 413–418.
    [Google Scholar]
  11. Souli M. , Kontopidou F. V. , Papadomichelakis E. , Galani I. , Armaganidis A. , Giamarellou H. . ( 2008;). Clinical experience of serious infections caused by Enterobacteriaceae producing VIM-1 metallo-β-lactamase in a Greek University Hospital. . Clin Infect Dis 46:, 847–854, [CrossRef] [PubMed].
    [Google Scholar]
  12. Tumbarello M. , Sanguinetti M. , Montuori E. , Trecarichi E. M. , Posteraro B. , Fiori B. , Citton R. , D'Inzeo T. , Fadda G. , other authors . ( 2007;). Predictors of mortality in patients with bloodstream infections caused by extended-spectrum-β-lactamase-producing Enterobacteriaceae: importance of inadequate initial antimicrobial treatment. . Antimicrob Agents Chemother 51:, 1987–1994, [CrossRef] [PubMed].
    [Google Scholar]
  13. Woodford N. , Eastaway A. T. , Ford M. , Leanord A. , Keane C. , Quayle R. M. , Steer J. A. , Zhang J. , Livermore D. M. . ( 2010;). Comparison of BD Phoenix, Vitek 2, and MicroScan automated systems for detection and inference of mechanisms responsible for carbapenem resistance in Enterobacteriaceae . . J Clin Microbiol 48:, 2999–3002, [CrossRef] [PubMed].
    [Google Scholar]
  14. Zavascki A. P. , Barth A. L. , Gonçalves A. L. , Moro A. L. , Fernandes J. F. , Martins A. F. , Ramos F. , Goldani L. Z. . ( 2006;). The influence of metallo-β-lactamase production on mortality in nosocomial Pseudomonas aeruginosa infections. . J Antimicrob Chemother 58:, 387–392, [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000067
Loading
/content/journal/jmm/10.1099/jmm.0.000067
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error