Mechanisms of azole resistance among clinical isolates of in Poland Free

Abstract

is currently ranked as the second most frequently isolated aetiological agent of human fungal infections, next only to . In comparison with , shows lower susceptibility to azoles, the most common agents used in treatment of fungal infections. Interestingly, the mechanisms of resistance to azole agents in have been much better investigated than those in . The aim of the presented study was to determine the mechanisms of resistance to azoles in 81 clinical isolates from three different hospitals in Poland. The investigation was carried out with a Sensititre Yeast One test and revealed that 18 strains were resistant to fluconazole, and 15 were cross-resistant to all other azoles tested (voriconazole, posaconazole and itraconazole). One isolate resistant to fluconazole was cross-resistant to voriconazole, and resistance to voriconazole only was observed in six other isolates. All strains were found to be susceptible to echinocandins and amphotericin B, and five were classified as resistant to 5-fluorocytosine. The sequence of the gene encoding lanosterol 14-α demethylase (the molecular target of azoles) of 41 isolates, including all strains resistant to fluconazole and three resistant only to voriconazole, was determined, and no amino acid substitutions were found. Real-time PCR studies revealed that 13 of 15 azole-resistant strains showed upregulation of the gene encoding the efflux pump. No upregulation of expression of the or gene was observed.

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2015-06-01
2024-03-29
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