1887

Abstract

The operon encoding polysaccharide intercellular adhesion, which facilitates biofilm formation in staphylococci, has been extensively studied in and . Based on analysis, we suggest the following functional model for Ica proteins in IcaA is responsible for polysaccharide synthesis. IcaA and IcaD complete transferring the growing sugar chain to the cell surface; IcaB is a deacetylase, with the same function as IcaB of . IcaC mainly modifies the synthesized glucan by acetylation. We also examined the effects of subinhibitory concentrations of erythromycin on phenotypic biofilm expression and transcription of biofilm-related genes, using isolates representing the two subspecies of and different biofilm and resistance phenotypes. On induction with erythromycin, biofilm density was strongly elevated in two erythromycin-resistant , but not in three susceptible isolates. In the representative erythromycin-resistant subsp. , there were significant upregulations of the gene and its positive regulator on transition to the stationary phase without erythromycin induction. There were also significant increases in the transcription levels of , and corresponding to a very strong biofilm phenotype in the stationary phase on erythromycin stress. In contrast, the representative erythromycin-susceptible subsp. displayed upregulation only of on entry into the stationary phase with erythromycin induction, but this change was not associated with enhancement of biofilm production. These findings suggest that the two subspecies of adopt different pathogenesis and survival strategies to adapt to a hostile environment.

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2015-06-01
2019-12-06
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