1887

Abstract

Considering the prevalence of infections in the Americas and the scarce information about the species, we aimed to apply a multilocus variable number tandem repeat (VNTR) analysis (MLVA) for the molecular typing of isolates from various sources. Amplification of three VNTR loci selected from genome sequences resulted in a wide range of sizes for the amplified products amongst the 21 strains analysed. This suggested a variation in tandem repeat copy numbers in the VNTR loci. sequencing also showed a high nucleotide diversity, confirming the MLVA data. In conclusion, this novel MLVA provided a high level of discrimination between isolates, and this new typing tool could be used to investigate leptospirosis in regions where predominates.

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2015-05-01
2021-03-07
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