1887

Abstract

Diarrhoea is one of leading causes of morbidity and mortality worldwide. Recent estimations suggested the number of deaths is close to 2.5 million. This study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of Khartoum, Sudan. A total of 437 stool samples obtained from children with diarrhoea were examined by culture and PCR for bacteria, by microscopy and PCR for parasites and by immunoassay for detection of rotavirus A. Of the 437 samples analysed, 211 (48 %) tested positive for diarrhoeagenic , 96 (22 %) for rotavirus A, 36 (8 %) for spp., 17 (4 %) for spp., 8 (2 %) for spp., 47 (11 %) for and 22 (5 %) for . All isolates of (211, 100 %) and (17, 100 %), and 30 (83 %) isolates of were sensitive to chloramphenicol; 17 (100 %) isolates of , 200 (94 %) isolates of and (78 %) 28 isolates of spp. were sensitive to gentamicin. In contrast, resistance to ampicillin was demonstrated in 100 (47 %) isolates of and 16 (44 %) isolates of spp. In conclusion, proved to be the main cause of diarrhoea in young children in this study, followed by rotavirus A and protozoa. Determination of diarrhoea aetiology and antibiotic susceptibility patterns of diarrhoeal pathogens and improved hygiene are important for clinical management and controlled strategic planning to reduce the burden of infection.

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2015-04-01
2019-10-20
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