1887

Abstract

complex is a significant pathogen in patients with non-cystic fibrosis (non-CF). Nevertheless, there is little description of the genetic diversity of this species. The aims of this study were to investigate the distribution of complex isolated from respiratory specimens by variable number tandem repeat (VNTR) typing. The results of 104 clinical isolates from 104 non-CF patients were compared using PFGE, genotypes and clarithromycin susceptibility. The allelic diversity (Hunter–Gaston Discriminatory Index) of the 17 loci examined by VNTR typing was high (0.977). We determined that C28 sequevar (41) genotypes and clarithromycin-acquired resistance isolates were scattered in the minimum spanning tree. Intriguingly, VNTR typing and PFGE were highly congruent and revealed that there were clear examples of grouping of isolates from different individuals amongst both and , and showed five clusters of distinct identical isolates. Within these clusters, type I formed three different clusters. Although the distribution of type II-1 was low (9.3 %), type II-1 isolates separated from clusters contained type I isolates. Thus, genotypes could be discriminated by analysing VNTRs with sufficient genetic distance for intra-species-level discrimination.

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2015-03-01
2024-03-29
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