1887

Abstract

Fast and reliable identification of bacteria to at least the species level is currently the basis for correct diagnosis and appropriate treatment of infections. This is particularly important in the case of bacteria of the genus , whose resistance profile is often correlated with their species (e.g. resistance to vancomycin). In this study, we evaluated restriction endonuclease analysis of the 16S–23S rRNA gene intergenic transcribed spacer (ITS) region for species identification of . The utility of the method was compared with that of phenotypic methods [biochemical profile evaluation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)]. Identification was based on 21 reference strains, of the species , , , , , , and , and 47 field strains isolated from pigs. Restriction endonuclease analysis of the ITS-PCR product using fI, I and I, in the order specified, enabled species differentiation of the reference and field strains, and in the case of the latter, the results of species identification were identical (47/47) to those obtained by MALDI-TOF MS. Moreover, as a result of digestion with I, a unique restriction profile was also obtained for the strains (3/3) identified by MALDI-TOF MS as In our opinion, restriction endonuclease analysis of the 16S–23S rRNA gene ITS region of may be a simple and relatively fast (less than 4 h) alternative method for identifying the species occurring most frequently in humans and animals.

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2015-03-01
2021-10-17
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