1887

Abstract

A novel Gram-positive, anaerobic and thermophilic bacterium, strain MET79, was isolated from an oil well located in the Gulf of Mexico. Cells were straight rods, motile by a subpolar flagellum. Spores were formed in old cultures. Inner gas vacuoles swelled the cells when exposed to air. The optimum growth conditions were 55 °C, pH 7·5 and 1 % NaCl. Yeast extract was required for growth. Strain MET79 fermented several sugars, some organic acids and Casamino acids. Glucose was fermented into lactate, acetate, butyrate, H and CO. Strain MET79 reduced thiosulfate to hydrogen sulfide and nitrate to ammonium. The DNA G+C content was 30·9 mol%. The closest phylogenetic relative of strain MET79 was (88·7 % 16S rDNA sequence similarity). As strain MET79 (=DSM 15102=CIP 107615) was physiologically and phylogenetically different from its closest relatives, it is assigned as the type strain of a novel species of a new genus, gen. nov., sp. nov.

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2003-09-01
2020-06-04
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