1887

Abstract

A novel alkaliphilic, sulfate-reducing bacterium, strain MLF1, was isolated from sediments of soda Mono Lake, California. Gram-negative vibrio-shaped cells were observed, which were 0·6–0·7×1·2–2·7 μm in size, motile by a single polar flagellum and occurred singly, in pairs or as short spirilla. Growth was observed at 15–48 °C (optimum, 37 °C), >1–7 % NaCl, w/v (optimum, 3 %) and pH 8·0–10·0 (optimum, 9·5). The novel isolate is strictly alkaliphilic, requires a high concentration of carbonate in the growth medium and is obligately anaerobic and catalase-negative. As electron donors, strain MLF1 uses hydrogen, formate and ethanol. Sulfate, sulfite and thiosulfate (but not sulfur or nitrate) can be used as electron acceptors. The novel isolate is a lithoheterotroph and a facultative lithoautotroph that is able to grow on hydrogen without an organic source of carbon. Strain MLF1 is resistant to kanamycin and gentamicin, but sensitive to chloramphenicol and tetracycline. The DNA G+C content is 63·0 mol% (HPLC). DNA–DNA hybridization with the most closely related species, Z-7951, exhibited 51 % homology. Also, the genome size (1·6×10 Da) and value of the genomic DNA (71±2 °C) for strain MLF1 were significantly different from the genome size (2·1×10 Da) and value (63±2 °C) for Z-7951. On the basis of physiological and molecular properties, the isolate was considered to be a novel species of the genus , for which the name sp. nov. is proposed (the type strain is MLF1=ATCC BAA-395=DSM 14708).

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2003-09-01
2020-06-04
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