1887

Abstract

Two airborne bacterial isolates designated V45 and V54A were characterized in order to determine their taxonomic position. 16S rDNA sequence analysis showed that the two isolates shared 98·1 % sequence similarity. Highest sequence similarities (98·0–98·5 %) were found to DSM 43110 and IFO 14650. Detection of a quinone system with the predominant compound MK-9(H), a polar lipid pattern containing phosphatidylglycerol, a fatty acid profile with the predominant acids C iso and C anteiso and the diagnostic cell-wall diamino acid -lysine supported the assignment of the novel isolates to the genus . The two isolates could be distinguished from by the presence of glycine in the peptidoglycan, and the detection of the cell-wall sugar galactose differentiates them from the two established species of the genus . Each of the two isolates displayed a unique biochemical profile. Results from DNA–DNA hybridizations clearly demonstrated that V45 and V54A represent separate species. Based on these data, it is proposed that V45 (=IFO 16525=CCM 7044) and V54A (=IFO 16526 =CCM 7043) be classified as the type strains of two novel species, for which the names sp. nov. and sp. nov. are proposed.

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2003-09-01
2020-06-04
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