1887

Abstract

An organism commonly referred to as ‘megabacterium’ colonizes the gastric isthmus of many species of birds. It is weakly Gram-positive and periodic acid–Schiff-positive and stains with silver stains. Previous studies have shown that it has a nucleus and a cell wall similar to those seen in fungi. Calcofluor white M2R staining suggests that the cell wall contains chitin, a eukaryote-specific substance, and rRNA hybridization demonstrates that it is a eukaryote. To characterize this organism phylogenetically, DNA was extracted from purified cells. rDNA was readily amplified by PCR with pan-fungal DNA primer sets and primer sets derived from the newly determined sequence, but not with bacteria-specific primer sets. Specific primer sets amplified rDNA from isthmus scrapings from an infected bird, but not from a non-infected bird or other control DNA. The sequence was confirmed to derive from the purified organism by rRNA hybridization using a specific probe. Phylogenetic analysis of sequences of the 18S rDNA and domain D1/D2 of 26S rDNA showed the organism to be a previously undescribed anamorphic ascomycetous yeast representing a new genus. The name gen. nov., sp. nov. is proposed for this organism. The type material is CBS 9251 (=NRRL Y-27487).

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2003-07-01
2020-06-04
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References

  1. Baker J. R. 1992; Megabacteriosis in exhibition budgerigars. Vet Rec 131:12–14 [CrossRef]
    [Google Scholar]
  2. Dorrestein G. M., Zwart P., Buitelaar M. N. 1980; Problems arising from disease during the periods of breeding and rearing canaries and other aviary birds. Tijdschr Diegeneesk 105:535–543
    [Google Scholar]
  3. Filippich L. J., Perry R. A. 1993; Drug trials against Megabacteria in budgerigars ( Melopsittacus undulatus . Aust Vet Pract 23:184–189
    [Google Scholar]
  4. Filippich L. J., Boyle D. A., Webb R., Fuerst J. A. 1993; Megabacteria in birds in Australia. Aust Vet Pract 23:71–76
    [Google Scholar]
  5. Gerlach H. 2001; Megabacteriosis. Semin Avian Exotic Pet Med 10:12–19 [CrossRef]
    [Google Scholar]
  6. Huchzermeyer F. W., Henton M. M., Keffen R. H. 1993; High mortality associated with megabacteriosis of proventriculus and gizzard in ostrich chicks. Vet Rec 133:143–144 [CrossRef]
    [Google Scholar]
  7. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331–371 [CrossRef]
    [Google Scholar]
  8. Monheit J. E., Cowan D. F., Moore D. G. 1984; Rapid detection of fungi in tissues using calcofluor white and fluorescence microscopy. Arch Pathol Lab Med 108:616–618
    [Google Scholar]
  9. Moore R. P., Snowden K. F., Phalen D. N. 2001; A method of preventing transmission of so-called ‘megabacteria’ in budgerigars ( Melopsittacus undulatus . J Avian Med Surg 15:283–287 [CrossRef]
    [Google Scholar]
  10. Mutlu O. F., Seckin S., Ravelhofer K., Hildebrand R.-A., Grimm F. 1997; Proventriculitis in domestic fowl ( Gallus gallus var. dom. L. 1758. caused by megabacteria. Tierärztl Prax 25:460–462
    [Google Scholar]
  11. Oliveira K., Haase G., Kurtzman C., Hyldig-Nielsen J. J., Stender H. 2001; Differentiation of Candida albicans and Candida dubliniensis by fluorescent in situ hybridization with peptide nucleic acid probes. J Clin Microbiol 39:4138–4141 [CrossRef]
    [Google Scholar]
  12. Pennycott T. W., Ross H. M., McLaren I. M., Park A., Hopkins G. F., Foster G. 1998; Causes of death of wild birds of the family Fringillidae in Britain. Vet Rec 143:155–158 [CrossRef]
    [Google Scholar]
  13. Ravelhofer K., Rotheneder R., Gareis M., Suttner R., Wolf O., Matiello R., Kösters J. 1998; Megabakteriosen bei verschiedenen Vogelspezies. In DVG Tagung über Vogelkrankheiten vol. 11: pp 95–104 Gießen: Deutsche Veterinärmedizinische Gesellschaft;
    [Google Scholar]
  14. Ravelhofer-Rotheneder K., Engelhardt H., Wolf O., Amann R., Breuer W., Kösters J. 2000; Taxonomic classification of “megabacteria” isolates originating from budgerigars ( Melopsittacus undulatus Shaw, 1805. Tierärztl Prax 28:415–420
    [Google Scholar]
  15. Relman D. A. 1993; The identification of uncultured microbial pathogens. J Infect Dis 168:1–8 [CrossRef]
    [Google Scholar]
  16. Sandhu G. S., Kline B. C., Stockman L., Roberts G. D. 1995; Molecular probes for diagnosis of fungal infections. J Clin Microbiol 33:2913–2919
    [Google Scholar]
  17. Scanlan C. M., Graham D. L. 1990; Characterization of a Gram-positive bacterium from the proventriculus of budgerigars ( Melopsittacus undulatus . Avian Dis 34:779–786 [CrossRef]
    [Google Scholar]
  18. Schulze C., Heidrich R. 2001; Megabakterien-assoziierte proventridulitis beim nutzgefügel in Brandenburg. Dtsch Tieräztl Wochenschr 108:264–266
    [Google Scholar]
  19. Swofford D. L. 1998 paup* 4.0: phylogenetic analysis using parsimony Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  20. Tomaszewski E., Wilson V. G., Wigle W. L., Phalen D. N. 2001; Detection and heterogeneity of herpesviruses causing Pacheco's disease in parrots. J Clin Microbiol 39:533–538 [CrossRef]
    [Google Scholar]
  21. van Herck H., Duijser T., Zwart P., Dorrestein G. M., Buitelaar M., van der Hage M. H. 1984; A bacterial proventriculitis of canaries. Avian Pathol 13:561–572 [CrossRef]
    [Google Scholar]
  22. White T. J., Bruns T., Lee S., Taylor J. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide to Methods and Applications pp 315–322 Philadelphia: Academic Press;
    [Google Scholar]
  23. Wieliczko A., Kuczkowski M. 2000; Selected issues of infectious diseases in ostrich ( Struthio camelus . Med Weter 56:23–28
    [Google Scholar]
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