1887

Abstract

Five isolates recovered from the respiratory tract of cystic fibrosis patients were included in a polyphasic taxonomic study that employed 16S rDNA sequence analysis, cellular protein and fatty acid analysis and biochemical characterization. Four isolates were classified as a novel species, for which the name sp. nov. is proposed; the other isolate was phylogenetically closely related to , but is likely to represent another novel species. The type strain of is AU3313 (=LMG 21510=CCUG 46809).

Keyword(s): CF, cystic fibrosis
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2003-09-01
2020-06-04
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References

  1. Burns J. L., Emerson J., Stapp J. R., Yim D. L., Krzewinski J., Louden L., Ramsey B. W., Clausen C. R. 1998; Microbiology of sputum from patients at cystic fibrosis centers in the United States. Clin Infect Dis 27:158–163 [CrossRef]
    [Google Scholar]
  2. Chen W.-M., Laevens S., Lee T.-M., Coenye T., De Vos P., Mergeay M., Vandamme P. 2001; Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51:1729–1735 [CrossRef]
    [Google Scholar]
  3. Coenye T., Falsen E., Vancanneyt M., Hoste B., Govan J. R. W., Kersters K., Vandamme P. 1999; Classification of Alcaligenes faecalis -like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49:405–413 [CrossRef]
    [Google Scholar]
  4. Coenye T., Goris J., Spilker T., Vandamme P., LiPuma J. J. 2002a; Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov. sp. nov. J Clin Microbiol 40:2062–2069 [CrossRef]
    [Google Scholar]
  5. Coenye T., Vandamme P., LiPuma J. J. 2002b; Infection by Ralstonia species in cystic fibrosis patients: identification of R. pickettii and R. mannitolilytica by polymerase chain reaction. Emerg Infect Dis 8:692–696 [CrossRef]
    [Google Scholar]
  6. Coenye T., Goris J., De Vos P., Vandamme P., LiPuma J. J. 2003; Classification of Ralstonia pickettii -like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov. Int J Syst Evol Microbiol 53:1075–1080 [CrossRef]
    [Google Scholar]
  7. De Baere T., Steyaert S., Wauters G., De Vos P., Goris J., Coenye T., Suyama T., Verschraegen G., Vaneechoutte M. 2001; Classification of Ralstonia pickettii biovar 3/‘ thomasii ’ strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov. Int J Syst Evol Microbiol 51:547–558
    [Google Scholar]
  8. Gilligan P. H. 1991; Microbiology of airway disease in patients with cystic fibrosis. Clin Microbiol Rev 4:35–51
    [Google Scholar]
  9. Gillis M., Van Van T., Bardin R. 7 other authors 1995; Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int J Syst Bacteriol 45:274–289 [CrossRef]
    [Google Scholar]
  10. Goris J., De Vos P., Coenye T. 7 other authors 2001; Classification of metal-resistant bacteria from industrial biotopes as Ralstonia campinensis sp. nov., Ralstonia metallidurans sp. nov. and Ralstonia basilensis Steinle et al . 1998 emend. Int J Syst Evol Microbiol 51:1773–1782 [CrossRef]
    [Google Scholar]
  11. Henry D. A., Mahenthiralingam E., Vandamme P., Coenye T., Speert D. P. 2001; Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex. J Clin Microbiol 39:1073–1078 [CrossRef]
    [Google Scholar]
  12. LiPuma J. J., Dulaney B. J., McMenamin J. D., Whitby P. W., Stull T. L., Coenye T., Vandamme P. 1999; Development of rRNA-based PCR assays for identification of Burkholderia cepacia complex isolates recovered from cystic fibrosis patients. J Clin Microbiol 37:3167–3170
    [Google Scholar]
  13. Liu L., Coenye T., Burns J. L., Whitby P. W., Stull T. L., LiPuma J. J. 2002; Ribosomal DNA-directed PCR for identification of Achromobacter ( Alcaligenes ) xylosoxidans recovered from sputum samples from cystic fibrosis patients. J Clin Microbiol 40:1210–1213 [CrossRef]
    [Google Scholar]
  14. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp 493–521Edited by Goodfellow M., O'Donnell A. G. J. Chichester, UK: Wiley;
    [Google Scholar]
  15. Sahin N., Isik K., Tamer A. U., Goodfellow M. 2000; Taxonomic position of “ Pseudomonas oxalaticus ” strain Ox14T (DSM 1105T) (Khambata and Bhat, 1953) and its description in the genus Ralstonia as Ralstonia oxalatica comb. nov. Syst Appl Microbiol 23:206–209 [CrossRef]
    [Google Scholar]
  16. Steinle P., Stucki G., Stettler R., Hanselmann K. W. 1998; Aerobic mineralization of 2,6-dichlorophenol by Ralstonia sp. strain RK1. Appl Environ Microbiol 64:2566–2571
    [Google Scholar]
  17. Vandamme P., Goris J., Coenye T., Hoste B., Janssens D., Kersters K., De Vos P., Falsen E. 1999; Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov. Int J Syst Bacteriol 49:663–669 [CrossRef]
    [Google Scholar]
  18. von Graevenitz A. 1995; Acinetobacter , Alcaligenes , Moraxella , and other nonfermentative Gram-negative bacteria. In Manual of Clinical Microbiology , 6th edn. pp 520–532Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: ASM Press;
    [Google Scholar]
  19. Yabuuchi E., Kosako Y., Yano I., Hotta H., Nishiuchi Y. 1995; Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov. Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol Immunol 39:897–904 [CrossRef]
    [Google Scholar]
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