1887

Abstract

Two Gram-positive or Gram-variable, endospore-forming, moderately halophilic rods (strains TF-11 and TF-12) were isolated from a tidal flat of the Yellow Sea in Korea and were subjected to a polyphasic taxonomic study. Strains TF-11 and TF-12 had cell-wall peptidoglycan based on -diaminopimelic acid. The predominant menaquinone found in the two strains was MK-7. The cellular fatty acid profiles of both strains contained large amounts of branched and saturated fatty acids. The major fatty acids were anteiso-C and iso-C. The DNA G+C contents of strains TF-11 and TF-12 were respectively 49 and 38 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strains TF-11 and TF-12 fell within the radiation of the cluster comprising species. The level of 16S rDNA sequence similarity between strains TF-11 and TF-12 was 98·3 %. Strains TF-11 and TF-12 exhibited levels of 16S rDNA sequence similarity of less than 96·0 and 96·3 %, respectively, to species. The mean level of DNA–DNA relatedness between the two strains was approximately 7 %. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains TF-11 and TF-12 should be placed in the genus as two distinct novel species, for which the names sp. nov. (type strain TF-11=KCCM 41588=JCM 11544) and sp. nov. (type strain TF-12=KCCM 41589=JCM 11545) are proposed.

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2003-09-01
2020-06-04
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