RT Journal Article SR Electronic(1) A1 Anderson, J. D. A1 Forshaw, H. L. A1 Adams, Monica A. A1 Gillespie, W. A. A1 Sellin, Margaret A.YR 1976 T1 The Relevance of Growth Rates in Urine to the Pathogenesis of Urinary-tract Infections Due to Micrococcus Subgroup 3 (Staphylococcus Saprophyticus Biotype 3) JF Journal of Medical Microbiology, VO 9 IS 3 SP 317 OP 323 DO https://doi.org/10.1099/00222615-9-3-317 PB Microbiology Society, SN 1473-5644, AB Summary A novobiocin-resistant “ biotype ” of Micrococcus subgroup 3 (Staphylococcus saprophyticus) is known to be a primary pathogen of the female urinary tract and to cause infections as severe as those produced by Escherichia coli. The growth characteristics of this virulent biotype were compared in vitro with those of other Micrococcaceae and of E. coli to determine whether rapid growth explains the virulence of the biotype. Nutrient broth was shown to have growth-supporting qualities that differed from those of urine and it was therefore unsuitable for these studies. In urine, the virulent biotype grew more slowly, had a longer lag period, and reached much lower final viable counts than did Escherichia coli. Surprisingly, the virulent biotype also grew more slowly and reached a lower final viable count than did several other Micrococcaceae isolated from the urinary tract of healthy women. Urine from women who had suffered a recent infection with the virulent biotype had growth-supporting properties similar to those of urine from healthy women. Experiments with filtered forestream urine suggested that urethral secretions do not contain a factor determining growth rates of this organism in urine. The possibility that virulent strains adapt to rapid growth in urine was excluded. No evidence was obtained that the virulent biotype inhibits the growth of other Micrococcaceae in urine. Ability to grow rapidly in urine does not therefore explain the virulence of novobiocin-resistant strains of subgroup-3 micrococci., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-9-3-317