1887

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Volume 9, Issue 2

Qualitative and quantitative methods for detecting staphylococcal epidermolytic toxin Free

Abstract

Summary

Concentrated supernates of cultures of 98 strains of were screened for the production of epidermolytic toxin by (1) biological tests in 3-day-old mice, (2) double-diffusion precipitation tests against specific antiserum, and (3) the appearance of characteristic protein bands on thin-layer-gel isoelectric focusing. Positive results were obtained in all three of these tests with supernates from 11 of these cultures; the same 11 strains, and no others, produced epidermal splitting when newborn mice were challenged with viable organisms. Of the 14 phage-group-II strains included in the survey, eight (58 %) produced epidermolytic toxin. Three toxinogenic strains belonged to phage groups other than group II.

A radial-immunodiffusion test employing antiserum to purified epidermolytic toxin proved satisfactory for measuring amounts of epidermolytic toxin in excess of 200 εg per ml. The results of immunodiffusion tests indicated that six of the 11 positive strains produced two serologically distinct forms of epidermolytic toxin and that the remainder produced only one of these. A striking correlation was observed between the presence of toxin of serotypes i and ii and the occurrence of protein bands i and ii in thin-layer isoelectric-focusing gels.

These tests should facilitate the qualitative and quantitative assessment of the production of different serotypes of epidermolytic toxin by in future surveys of strains isolated from toxic epidermal necrolysis of Ritter's type and impetigo.

The two forms of epidermolytic toxin previously designated ET (I = 7-0) and ET (I = 6η0) were detected by preparative isoelectric focusing of sero-type-i toxin. Evidence suggests that studies of the effect of heat should provide a means of investigating the relationship between the different molecular forms of epidermolytic toxin.

  • Received:
  • Accepted:
  • Published Online:
© The Pathological Society of Great Britain and Ireland, 1976
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References

  1. Arbuthnott J. P., Billcliffe B., Thompson W. D. 1974; Isoelectric focusing studies of staphylococcal epidermolytic toxin. FEBS Letts. 46:92
     [Google Scholar]
  2. Arbuthnott J. P., Kent J., Lyell A., Gemmell C. G. 1971; Toxic epidermal necrolysis produced by an extracellular product of Staphylococcus aureus . Br. J. Derm. 85:145
     [Google Scholar]
  3. Arbuthnott J. P., Kent J., Lyell A., Gemmell C. G. 1972; Studies of staphylococcal toxins in relation to toxic epidermal necrolysis (the scalded skin syndrome). Br. J. Derm. 86:Suppl8–35
     [Google Scholar]
  4. Arbuthnott J. P., Kent J., Noble W. C. 1973; The response of hairless mice to staphylococcal epidermolytic toxin. Br. J. Derm. 88:481
     [Google Scholar]
  5. Bernheimer A. W., Schwartz L. L. 1963; Isolation and composition of staphylococcal oc-toxin. J. gen. Microbiol. 30:455
     [Google Scholar]
  6. Davies H. 1975 Thin-layer gel isoelectric focusing. In Isoelectric focusing. edited by Arbuthnott J. P., Beeley J. A. London: p 97
     [Google Scholar]
  7. Elias P. M., Fritsch P., Tappeiner G., Mittermayer H., Wolff K. 1974a; Experimental staphylococcal toxic epidermal necrolysis (TEN) in adult humans and mice. J. Lab. clin. Med. 84:414
     [Google Scholar]
  8. Elias P. M., Mittermayer H., Tappeiner G., Fritsch P., Wolff K. 1974b; Staphylococcal toxic epidermal necrolysis (TEN): the expanded mouse model. J. invest. Derm. 63:467
     [Google Scholar]
  9. Kapral F. A. 1974; Staphylococcus aureus: some host-parasite interactions. Ann. N. Y. Acad. Sci. 236:267
     [Google Scholar]
  10. Kapral F. A., Miller M. M. 1971; Product of Staphylococcus aureus responsible for the scalded skin syndrome. Infect. Immun. 4:541
     [Google Scholar]
  11. Kapral F. A., Miller M. M. 1972; Skin lesions produced by Staphylococcus aureus exfoliatin in hairless mice. Infect. Immun. 6:877
     [Google Scholar]
  12. Kondo I., Sakurai S., Sarai Y. 1973; Purification of exfoliatin produced by Staphylococcus aureus of bacteriophage group 2 and its physicochemical properties. Infect. Immun. 8:156
     [Google Scholar]
  13. Kondo I., Sakurai S., Sarai Y. 1974; New type of exfoliatin obtained from staphylococcal strains belonging to phage groups other than group II isolated from patients with impetigo and Ritter’s disease. Infect. Immun. 10:851
     [Google Scholar]
  14. Kondo I., Sakurai S., Sarai Y., Futaki S. 1975; Two serotypes of exfoliatin and their distribution in staphylococcal strains isolated from patients with scalded skin syndrome. J. clin. Microbiol. 1:397
     [Google Scholar]
  15. Lillibridge C. B., Melish M. E., Glasgow L. A. 1972; Site of action of exfoliative toxin in the staphylococcal scalded-skin syndrome. Pediatrics, Springfield 50:728
     [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265
     [Google Scholar]
  17. McCallum H. M. 1972; Action of staphylococcal epidermolytic toxin on mouse skin in organ culture. Br. J. Derm. 86:Suppl. 840
     [Google Scholar]
  18. McLay A. L. C., Arbuthnott J. P., Lyell A. 1975; Action of staphylococcal epidermolytic toxin on mouse skin: an electron microscopic study. J. invest. Derm. 65:423
     [Google Scholar]
  19. McNiven A. C., Owen P., Arbuthnott J. P. 1972; Multiple forms of staphylococcal 03B1-toxin. J. med. Microbiol. 5:113
     [Google Scholar]
  20. Melish M. E., Glasgow L. A. 1970; The staphylococcal scalded skin syndrome: development of an experimental model. New. Engl. J. Med. 282:1114
     [Google Scholar]
  21. Melish M. E., Glasgow L. A., Turner M. D. 1972; The staphylococcal scalded skin syndrome: isolation and partial characterization of the exfoliative toxin. J. infect. Dis. 125:129
     [Google Scholar]
  22. Miles A. A., Misra S. S., Irwin J. O. 1938; The estimation of the bactericidal power of blood. J. Hyg., Camb. 38:732
     [Google Scholar]
  23. Vesterberg O. 1972; Isoelectric focusing of proteins in polyacrylamide gels. Biochim. biophys. Acta. 257:11
     [Google Scholar]
  24. Wiley B. B., Allman S., Rogolsky M., Norden C. W., Glasgow L. A. 1974; Staphylococcal scalded skin syndrome: potentiation by immunosuppression in mice: toxin mediated exfoliation in a healthy adult. Infect. Immun. 9:636
     [Google Scholar]
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Qualitative and quantitative methods for detecting staphylococcal epidermolytic toxin
J. Med. Microbiol. 9, 191 (1976); https://doi.org/10.1099/00222615-9-2-191
/content/journal/jmm/10.1099/00222615-9-2-191
/content/journal/jmm/10.1099/00222615-9-2-191
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