1887

Abstract

Summary

Synthesis of α-haemolysin by was proportional to the amount of meat-broth factor present in the medium and not to bacterial growth.

The meat-broth component required for the synthesis of haemolysin was found to have several physical and chemical properties in common with α-haemolysin itself. Both molecules are trypsin-sensitive, acidic substances with similar elution volumes when subjected to gel-filtration chromatography with Sepharose 6B.

Isotopic labelling experiments in which the bacteria were grown on C-labelled meat broth showed that partially purified haemolysin preparations contained molecules of mammalian origin. Similar experiments in which C-glucose was used to label bacterial molecules showed that little or no material of bacterial origin was present in the haemolysin preparation.

These data suggest that α-haemolysin may be produced by bacterial modification of a molecule present in meat broth rather than by synthesis within the bacterial cell.

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1976-05-01
2024-12-09
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References

  1. Alouf J. E., Raynaud M. 1970 Isolation and purification of bacterial toxic proteins. In Microbial toxins. edited by Ajl S. J., Kadis S., Montle T. C. New York: vol 1: p 119
    [Google Scholar]
  2. Bernheimer A. W. 1970 Cytolytic toxins of bacteria. In Microbial toxins. edited by Ajl S. J., Kadis S., Montie T. C. New York,: vol 1: p. 183
    [Google Scholar]
  3. Bernheimer A. W., Grushoff P. 1967; Cereolysin: production, purification, and partial characterization. J. gen. Microbiol. 46:143
    [Google Scholar]
  4. Bonventre P. F., Johnson C. E. 1970 Bacillus cereus toxin. In Microbial toxins. edited by Montie T. C., Kadis S., Ajl S. J. New York: vol 3 p. 415
    [Google Scholar]
  5. Chaturvedi U. C., Mathur A., Khan A. M., Mehrotra R. M. L. 1969; Cytotoxicity of filtrates of haemolytic Escherichia coli . J. med. Microbiol 2:211
    [Google Scholar]
  6. DePauw A. p., Gill W. B., Fried F. A. 1971; Etiology of pyelonephritis: renal lysosome disruption by hemolytic Escherichia coli . Invest. Urol. 9:230
    [Google Scholar]
  7. Fraser D., Jerrell E. A. 1953; The amino acid composition of T3 bacteriophage. J. biol. Chem 205:291
    [Google Scholar]
  8. Goebel W., Royer-Pokora B., Lindenmaier W., Bujard H. 1974; Plasmids controlling synthesis of hemolysin in Escherichia coli: molecular properties. J. Bact. 118:964
    [Google Scholar]
  9. Goebel W., Schrempf H. 1971; Isolation and characterization of supercoiled circular deoxyribonucleic acid from beta-hemolytic strains of Escherichia coli . J. Bact. 106:311
    [Google Scholar]
  10. Inukai Y., Kodama H. 1965; Studies on hemolytic Escherichia coli of 0-139; a certain factor essential for hemolysin production. Jap. J. vet. Res. 13:87
    [Google Scholar]
  11. Kato I. 1970 Relationship of bacterial structure and metabolism to toxin production. In Microbial toxins. edited by Ajl S. J., Kadis S., and Montie T. C. New York,: vol 1: p. 401
    [Google Scholar]
  12. Kurtz H. J., Bergeland M. E., Barnes D. M. 1969; Pathologic changes in edema disease of swine. Am. J. vet. Res. 30:791
    [Google Scholar]
  13. Lovell R., Rees T. A. 1960; A filterable hemolysin from Escherichia coli . Nature, Lond. 188:755
    [Google Scholar]
  14. Muranyi F., Juhasz S. 1971; Haemolytic effect of Escherichia coli cultures and their filtrates. Acta vet. hung. 21:355
    [Google Scholar]
  15. Pollock M. R. 1962 Exoenzymes. In The bacteria. edited by Gunsalus I. C., and Stanier R. Y. New York,: vol 4: p. 121
    [Google Scholar]
  16. Raynaud M., Alouf J. E. 1970 Intracellular versus extracellular toxins. In Microbial toxins. edited by Ajl S. J., Kadis S., and Montie T. C. New York,: vol 1: p. 67
    [Google Scholar]
  17. Rennie R. P., Arbuthnott J. P. 1971; Effect of carbohydrates on hemolysin production by Escherichia coli . Infect. Immun. 3:849
    [Google Scholar]
  18. Rennie R. P., Arbuthnott J. P. 1974; Partial characterisation of Escherichia coli haemolysin.. J. med. Microbiol 7:179
    [Google Scholar]
  19. Roberts R. B., Cowie D. B., Abelson P. H., Bolton E. T., Britten R. J. 1955 Studies of biosynthesis in Escherichia coli. Carnegie Institution of Washington; publication no. 607 Washington, D.C.: p. 13
    [Google Scholar]
  20. Short E. C., Kurtz H. J. 1971; Properties of the hemolytic activities of Escherichia coli . Infect. Immun. 3:678
    [Google Scholar]
  21. Smith H. W. 1963; The haemolyins of Escherichia coli . J. Path. Bact. 85:197
    [Google Scholar]
  22. Smith H. W., Halls S. 1967; The transmissible nature of the genetic factor in Escherichia coli that controls haemolysin production. J. gen. Microbiol. 41:153
    [Google Scholar]
  23. Snyder I. S., Koch N. A. 1966; Production and characteristics of hemolysins of Escherichia coli . J. Bact 91:763
    [Google Scholar]
  24. Snyder I. S., Zwadyk P. 1969; Some factors affecting the production and-assay of Escherichia coli haemolysins. J. gen. Microbiol. 55:139
    [Google Scholar]
  25. Walton J. R., Smith D. H. 1969; New hemolysin (γ) produced by Escherichia coli . J. Bact. 98:304
    [Google Scholar]
  26. Zwadyk P., Jr, Snyder I. S. 1971; Purification and kinetic studies of the hemolysin from Escherichia coli. Can . J. Microbiol. 17:741
    [Google Scholar]
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