1887

Abstract

Summary

The interactions of a contractile, a filamentous and a small pyocine with a sensitive strain of (no. P14) were examined The purification procedure used yielded high-activity pyocine preparations that were not toxic to mice. The inhibitory activity of such preparations, when injected into mice by various routes, was retained for up to 24 h. However, high molecular-weight pyocines given intraperitoneally in the presence of a lethal dose of strain PI4 administered by the same route did not prevent the fatal outcome of infection unless they are given before or together with the bacteria. The small pyocine had no protective effect.

In burned mice infected with strain PI4, topical application of a filamentous pyocine failed to improve the chances of survival.

The results suggest that there is little future for pyocine therapy.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-9-2-153
1976-05-01
2024-11-11
Loading full text...

Full text loading...

/deliver/fulltext/jmm/9/2/medmicro-9-2-153.html?itemId=/content/journal/jmm/10.1099/00222615-9-2-153&mimeType=html&fmt=ahah

References

  1. Bird T. J., Grieble H. G. 1969; Pyocin antibiosis in chick embryos. Antimicrob. Agents Chemother 1969:495
    [Google Scholar]
  2. Brown D. O. 1973; Filamentous pyocines of Pseudomonas aeruginosa . FIMLT Thesis
    [Google Scholar]
  3. Gillies R. R., Govan J. R. W. 1966; Typing of Pseudomonas pyocyanea by pyocine production. J. Path. Bact. 91:339
    [Google Scholar]
  4. Govan J. R. W. 1974a; Studies on the pyocins of Pseudomonas aeruginosa: morphology and mode of action of contractile pyocins. J. gen. Microbiol. 80:1
    [Google Scholar]
  5. Govan J. R. W. 1974b; Studies on the pyocins of Pseudomonas aeruginosa: production of contractile and flexuous pyocins in Pseudomonas aeruginosa . J. gen. Microbiol. 80:17
    [Google Scholar]
  6. Higerd T. B., Baechler C. A., Berk R. S. 1967; In-vitro and in-vivo characterization of pyocin. J. Bact. 93:1976
    [Google Scholar]
  7. Ito S., Kageyama M., Egami F. 1970; Isolation and characterization of pyocins from several strains of Pseudomonas aeruginosa . J. gen. appl. Microbiol., Tokyo 16:205
    [Google Scholar]
  8. Jones R. J., Jackson D. McG, Lowbury E. J. L. 1966; Antiserum and antibiotic in the prophylaxis of bums against Pseudomonas aeruginosa . Br. J. plast. Surg. 19:43
    [Google Scholar]
  9. Kageyama M. 1964; Studies of a pyocin. I. Physical and chemical properties. J. Biochem., Tokyo 55:49
    [Google Scholar]
  10. Kageyama M., Egami F. 1962; On the purification and some properties of a pyocin, a bacteriocin produced by Pseudomonas aeruginosa . Life Sciences 9:471
    [Google Scholar]
  11. Merrikin D. J., Terry C. S. 1972; Use of pyocin 78-C2 in the treatment of Pseudomonas aeruginosa infection in mice. Appl. Microbiol 23:164
    [Google Scholar]
  12. Ohkawa I., Kageyama M., Egami F. 1973; Purification and properties of pyocin S2. J. Biochem., Tokyo 73:281
    [Google Scholar]
  13. Takeya K., Minamishima Y., Ohnishi Y., Amako K. 1969; Rod-shaped pyocin 28. J. gen. Virol. 4:145
    [Google Scholar]
  14. Williams R. J. 1974; Pyocines and the treatment of Pseudomonas aeruginosa infections. Ph.D. Thesis, University of Edinburgh
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-9-2-153
Loading
/content/journal/jmm/10.1099/00222615-9-2-153
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error