1887

Abstract

Mutants lacking flagella or fimbriae (pili) or both were used for the preparation and absorption of rabbit antisera against the flagella of Pseudomonas aeruginosa. The results of agglutination, immobilisation and complementfixation tests indicate that the antisera obtained are specific for flagella.

The incorporation of nitrate into semi-solid agar for motility and immobilisation tests was found useful for the selection of actively motile cells and for the demonstration of specific anti body to flagella.

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1975-02-01
2024-12-07
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References

  1. Aoki K. 1926; Agglutinatorische Einteilung von Pyocyaneus-Bazillen welche bei verschiedenen Menschenerkrankungen nachgeweisen wurden. Zentbl. Bakt. ParasitKde I. Abt. Orig 98:186
    [Google Scholar]
  2. Bradley D. E. 1972; Stimulation of pilus formation in Pseudomonas aeruginosa RNA bacteriophage adsorption. Biochem. biophys. Res. Commun 47:1080
    [Google Scholar]
  3. Bradley D. E. 1973; Basic characterisation of a Pseudomonas aeruginosa pilus-dependent bacteriophage with a long noncontractile tail. J. Virol 12:1139
    [Google Scholar]
  4. Bradley D. E., Pitt T. L. 1974; Pilus-dependence of four Pseudomonas aeruginosa bacteriophages with noncontractile tails. J. gen. Virol 23:1
    [Google Scholar]
  5. Bradstreet C. M. P., Taylor C. E. D. 1962; Technique of complement-fixation test applicable to the diagnosis of virus diseases. Mon. Bull Minist. Hlth 21:96
    [Google Scholar]
  6. Christie R. 1948; Observations on the biochemical and serological characteristics of Pseudomonas pyocyanea. Aust. J. exp. Biol. med. Sci 26:425
    [Google Scholar]
  7. Craigie J. 1931; Studies on the serological reactions of the flagella of B. typhosus. J. Immun 21:417
    [Google Scholar]
  8. Ende M. van den. 1952; Observations on the antigenic structure of Pseudomonas aeruginosa. J. Hyg., Camb 50:405
    [Google Scholar]
  9. Gaby W. L. 1946; A study of the dissociative behaviour of Pseudomonas aeruginosa. J. Bact 51:217
    [Google Scholar]
  10. Habs I. 1957; Untersuchungen liber die O-Antigene von Pseudomonas aeruginosa. Z. Hyg. InfektKrankh 144:218
    [Google Scholar]
  11. Holloway B. W. 1955; Genetic recombination in Pseudomonas aeruginosa. J. gen. Microbiol 13:572
    [Google Scholar]
  12. Homma J. Y., Kim K. S., Yamada H., Ito M., Shionoya H., Kawabe Y. 1970; Serological typing of Pseudomonas aeruginosa and its cross-infection. Jap. J. exp. Med 40:347
    [Google Scholar]
  13. Iino T. 1969; Genetics and chemistry of bacterial flagella. Bact. Rev 33:454
    [Google Scholar]
  14. Lányi B. 1966–67; Serological properties of Pseudomonas aeruginosa. I. Group-specific somatic antigens. Acta, microbiol. hung 13:295
    [Google Scholar]
  15. Lányi B. 1970; Serological properties of Pseudomonas aeruginosa. II. Type-specific thermolabile (flagellar) antigens. Acta, microbiol. hung 17:35
    [Google Scholar]
  16. Lawn A. M. 1967; Simple immunological labelling method for electron microscopy and its application to the study of filamentous appendages of bacteria. Nature, Lond 214:1151
    [Google Scholar]
  17. Matsui N. 1959; A serological study of Pseudomonas aeruginosa. J. Kansai. med. Sch 11:287
    [Google Scholar]
  18. Mayr-Harting A. 1948; The serology of Pseudomonas pyocyanea. J. gen. Microbiol 2:31
    [Google Scholar]
  19. Munoz J., Scherago M., Weaver R. H. 1945; A serological study of the members of the Pseudomonas genus. J. Bact 49:524
    [Google Scholar]
  20. Robinson G. L. 1932; The growth of B. pyocyaneus in synthetic media. Br. J. exp. Path 13:310
    [Google Scholar]
  21. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J. gen. Microbiol 43:159
    [Google Scholar]
  22. Verder E., Evans J. 1961; A proposed antigenic schema for the identification of strains of Pseudomonas aeruginosa. J. infect. Dis 109:183
    [Google Scholar]
  23. Weiss R. J., Raj H. D. 1972; The structure and isolation of pili (fimbriae) of Pseudomonas aeruginosa. Aust. J. exp. Biol. med. Sci 50:559
    [Google Scholar]
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