1887

Abstract

A solid, urea-containing medium buffered to pH 6.5 with a suitable mixture of KH2P04 and Na2HP04 produced enlarged T-mycoplasma colonies containing a white precipitate. This was absent from M. horninis colonies. The medium can be used for the isolation and identification of T-mycoplasmas.

We would like to thank Mr A. E. Pink for assistance with the analyses and Mr E. J. Kentish for the photography.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-8-1-183
1975-02-01
2022-05-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/8/1/medmicro-8-1-183.html?itemId=/content/journal/jmm/10.1099/00222615-8-1-183&mimeType=html&fmt=ahah

References

  1. Braun P., Klein J. D., Lee Y. H., Kass E. H. 1970; Methodologic investigations and prevalence of genital mycoplasmas in pregnancy. J. infect. Dis 121:391
    [Google Scholar]
  2. Edward D. G. FF. 1947; A selective medium for pleuropneumonia-like organisms. J. gen. Microbiol 1:238
    [Google Scholar]
  3. Friedlander A. M., Braude A. I. 1974; Production of bladder stones by human T-mycoplasmas. Nature, Lond 247:67
    [Google Scholar]
  4. Lee Y. H., Bailey P. E., Mccormack W. M. 1972; T-mycoplasmas from urine and vaginal specimens: decreased rates of isolation and growth in the presence of thallium acetate. J. infect. Dis 125:318
    [Google Scholar]
  5. Manchee R. J., Taylor-Robinson D. 1969; Enhanced growth of T-strain mycoplasmas with N-2-hydroxethyl-piperazine-N-2-ethanesulfonic acid buffer. J. Bact 100:78
    [Google Scholar]
  6. Michelsen O. B. 1957; Photometric determination of phosphorus as molybdovanado-phosphoric acid. Analyt. Chem 29:60
    [Google Scholar]
  7. Shepard M. C. 1969; Fundamental biology of the T-strains. In The Mycoplasmatales and the L-phase of bacteria edited by Hayflick L. New York: p 49
    [Google Scholar]
  8. Shepard M. C. 1973; Differential methods for identification of T-mycoplasmas based on demonstration of urease. J. infect. Dis 127: Suppl S22
    [Google Scholar]
  9. Shepard M. C., Lunceford C. D. 1970; Differential agar medium for identification of T-mycoplasmas in primary cultures. Bact. Proc p 83
    [Google Scholar]
  10. Shepard M. C., Lunceford C. D., Ford D. K., Purcell R. H., Taylor-Robinson D., Razin S., Black F. T. 1974; Ureaplasma urealyticum gen. nov., sp. nov: proposed nomenclature for the human T (T-strain) mycoplasmas. Int. J. syst. Bact 24:160
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-8-1-183
Loading
/content/journal/jmm/10.1099/00222615-8-1-183
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error