1887

Abstract

Immunodiffusion analysis of Mycobacterium lepraernurium indicated the presence of at least six antigens. Comparative analysis of the M. lepraemuriurn antigen-antibody system with similar systems established for other mycobacterial species, showed that M. lepraemurium shared up to two antigens with other species.

Although our observations are in accord with some of the studies on the antigenic mosaic of M. lepraemurium, they are in disagreement with the observations of Stanford (1973) concerning a close serological relationship of this organism to M. avium. This incompatibility cannot be explained satisfactorily at present.

We wish to thank Dr B. S. Tepper of the Johns Hopkins-Leonard Wood Memorial Leprosy Research Laboratory, Baltimore, Maryland, for supplying M. lepraemurium, Dr R. Merkal of the National Animal Diseases Laboratory, US Department of Agriculture, Ames, Iowa, for supplying the M. ohnei and Dr L. Levy of the United States Public Health Service Hospital, San Francisco, for the other mycobacterial strains used in this study. Our thanks are also due to the US-Japan Tuberculosis Panel’s Antigen Committee for supplying the M. tuberculosis (H37Rv) reference system.

This investigation was supported by the United States-Japan Cooperative Medical Sciences Program administered by the National Institutes of Allergy and Infectious Diseases of the National Institutes of Health, Education and Welfare (grant no. AI-0864-7), Bethesda, Maryland 20014, USA.

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1975-02-01
2022-01-22
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References

  1. Chang Y. T., Anderson R. N., Vaituzis Z. 1967; Growth of Mycobacterium lepraemurium in cultures of mouse peritoneal macrophage. J. Bact 93:1119
    [Google Scholar]
  2. Fukui Y., Mori T., Kohsaka K., Nishimura S., Yoneda M. 1966; Antigenic structure of Mycobacterium lepraemurium. 1. Presence of avian type antigen in strains of M. lepraemurium. Biken J 9:63
    [Google Scholar]
  3. Hanson L. A. 1959; Immunologic analysis of streptoccal antigens and human sera by means of diffusion-in-gel methods. Int. Archs Allergy appl. Immun 14:270
    [Google Scholar]
  4. Janicki B. W., Chaparas S. D., Daniel T. M., Kubica G. P., Wright G. L., Yee G. S. 1971; A reference system for antigens of Mycobacterium tuberculosis. Am. Rev. resp. Dis 104:602
    [Google Scholar]
  5. Kwapinski J. B. G., Alcasid A., Kwapinski E. H., Nairn V. 1972; The immunology of cytoplasmic antigens of mycobacteria. Can. J. Microbiol 18:1201
    [Google Scholar]
  6. Navalkar R. G. 1971; Immunologic analysis of Mycobacterium leprae antigens by means of diffusion-in-gel methods. Int. J. Lepr 39:105
    [Google Scholar]
  7. Navalkar R. G., Norlin M., Ouchterlony Ö. 1964; Characterization of leprosy sera with various mycobacterial antigens using double diffusion-in-gel analysis. Int. Archs Allergy appl. Immun 25:103
    [Google Scholar]
  8. Norlin M. 1965; Unclassified mycobacteria. A comparison between a serological and biochemical classification method. Bull. Un. int. Tuberc 36:25
    [Google Scholar]
  9. Ouchterlony Ö. 1962; Diffusion-in-gel methods for immunologic analysis II. Prog. Allergy 6:30
    [Google Scholar]
  10. Rees R. J. W., Garbutt E. W. 1962; Studies on Mycobacterium lepraemurium in tissue culture. 1. Multiplication and growth characteristics in cultures of rat fibroblasts. Br. J. exp. Path 43:221
    [Google Scholar]
  11. Rees R. J. W., Tee R. D. 1962; Studies on Mycobacterium lepraemurium in tissue culture. II. The production and properties of soluble antigens from Mycobacterium lepraemurium in tissue culture. Br. J. exp. Path 43:480
    [Google Scholar]
  12. Rees R. J. W., Wong P. C. 1958; Limited multiplication of Mycobacterium lepraemurium in tissue culture. Nature, Lond 181:359
    [Google Scholar]
  13. Sauton M. B. 1912; Sur la nutrition minéral du bacille tuberculeux. C.r. hebd. Séanc. Acad. Sci., Paris 155:860
    [Google Scholar]
  14. Stanford J. L. 1973; An immunodiffusion analysis of Mycobacterium lepraemurium Marchoux and Sorel. J. med. Microbiol 6:435
    [Google Scholar]
  15. Tepper B. S., Varma K. G. 1972; Metabolic activity of purified suspensions of Mycobacterium lepraemurium. J. gen. Microbiol 73:143
    [Google Scholar]
  16. Wallace J. H., Elek S. D., Hanks J. H. 1958; Limited multiplication of Mycobacterium lepraemurium in cell cultures. Proc. Soc. exp. Biol. Med 97:101
    [Google Scholar]
  17. Wayne L. G., Gross W. M. 1968; Base composition of deoxyribonucleic acid isolated from mycobacteria. J. Bact 96:1915
    [Google Scholar]
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