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A single-step disk immunoimmobilistion method for the detection of motile salmonellae is described. It combines in a single step the use of the motility of these bacteria in soft agar, their selective growth on Salmonella-Shigella media, and their immobilisation by polyvalent H antiserum. The method is shown to be 10 to 10,000 times more sensitive than a standard method for detection of Salmonella under the experimental conditions used. The time required for detection is shortened to 24-48 h.
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