1887

Abstract

A single-step disk immunoimmobilistion method for the detection of motile salmonellae is described. It combines in a single step the use of the motility of these bacteria in soft agar, their selective growth on Salmonella-Shigella media, and their immobilisation by polyvalent H antiserum. The method is shown to be 10 to 10,000 times more sensitive than a standard method for detection of Salmonella under the experimental conditions used. The time required for detection is shortened to 24-48 h.

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/content/journal/jmm/10.1099/00222615-8-1-173
1975-02-01
2024-04-25
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References

  1. Abrahamsson K., Patterson G., Riemann H. 1968; Detection of Salmonella by a single culture technique. Appl. Microbiol 16:1695
    [Google Scholar]
  2. Breed R. S., Murray E. G. D., Smith N. R. 1957; Bergey’s manual of determinative bacteriology, 7th ed.. Baltimore p 357
    [Google Scholar]
  3. Edwards P. R., Ewing W. H. 1962; Identification of Enterobacteriaceae. Minneapolis p 92
    [Google Scholar]
  4. Georgeala D. L., Boothroyd M. 1964; A rapid immunofluorescence technique for detecting Salmonella in raw meat. J. Hyg., Camb 62:319
    [Google Scholar]
  5. Haglund J. R., Ayres J. C., Patow A. M., Kraft A. A., Quinn L. Y. 1964; Detection of Salmonella in egg and egg products with fluorescent antibody. Appl. Microbiol 12:447
    [Google Scholar]
  6. Ino J., Graber C. D. 1955; Recovery of Salmonella from contaminated cultures. U.S. arm. Forces med. J 6:586
    [Google Scholar]
  7. Insalata N. F., Schulte S. J., Berman J. H. 1967; Immunofluorescence technique for detection of Salmonella in various foods. Appl. Microbiol 15:1145
    [Google Scholar]
  8. Mohit B. 1968; Disc immuno-immobilization method for simultaneous typing and isolation of Salmonella flagellar phases. J. Bact 96:160
    [Google Scholar]
  9. Raj H. 1966; Enrichment medium for selection of Salmonella from fish homogenate. Appl. Microbiol 14:12
    [Google Scholar]
  10. Rappaport F., Konforti N., Navon B. 1956; New enrichment medium for certain Salmonellae. J. clin. Path 9:261
    [Google Scholar]
  11. Silliker J. H., Diebel R. H., Cmu J. Y. 1964; Occurrence of Gram positive organisms possessing characteristics similar to those of Salmonella and practical problem of rapid and definitive Salmonella identification. Appl. Microbiol 12:395
    [Google Scholar]
  12. Silliker J. H., Fagan P. T., Chiu J. Y., Williams A. 1965; Polyvalent H agglutination as rapid means of screening non-lactose-fermenting colonies for Salmonella organisms. Am. J. clin. Path 43:548
    [Google Scholar]
  13. Silliker J. H., Schmall A., Chiu J. Y. 1966; The fluorescent antibody technique as a means of detecting Salmonella in foods. J. Fd Sci 31:240
    [Google Scholar]
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