1887

Abstract

SUMMARY

The vegetative growth of tetanus bacilli was examined in mice challenged intramuscularly with spores suspended in 4% CaCI solution. The viable count of organisms in material derived by grinding up the injection site was low at 6 hours and thereafter increased to counts of the order of 2×10 colony-forming units of in the ground-up lesion about 24 hours after inoculation. At that time the lesion volume was only about 1 ml.

In mice challenged with 1200 to 120,000 spores or in those given 2 MLD of toxin, the time taken for the resulting ascending tetanus to progress from its first appearance to become severe enough to prove fatal was similar-about 17 hours. For the infected mice and those challenged with toxin, however, the time of onset of the first signs of tetanus differed: it was 13 hours after inoculation of toxin, but 20 hours after injection of spores. It is suggested that the difference represents the time taken for the injected spores to form 2 MLD of toxin in the mouse, i.e., about 7 hours after challenge. At this time the numbers of viable organisms at the site of infection had not detectably increased, but rapid growth was about to begin.

These observations suggest that a lethal dose of tetanus toxin may be produced rapidly in an infected tissue by a very small number of viable organisms. Within 24 hours of challenge, a heavy growth of may be present in a lesion only about 1 ml in volume.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-7-4-497
1974-11-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/7/4/medmicro-7-4-497.html?itemId=/content/journal/jmm/10.1099/00222615-7-4-497&mimeType=html&fmt=ahah

References

  1. Booth R., Suzuki J. B., Berg B. E., Grecz N. 1972; Influence of weight and sex of mice in assaying spore-bound Clostridium botulinum type A toxin. J. Fd Sci 37:183
    [Google Scholar]
  2. Fildes P. 1927; Tetanus. VI The conditions under which tetanus spores germinate in vivo. Br. J. exp. Path 8:387
    [Google Scholar]
  3. Francis E. 1914; Laboratory studies on tetanus. Hygiene Laboratory Bulletin no. 95 U.S. Public Health Service; Washington:
    [Google Scholar]
  4. Keppie J. 1951; The pathogenicity of the spores of Clostridium botulinum. J. Hyg., Camb 49:36
    [Google Scholar]
  5. Russell D. S. 1927; Tetanus. V. The local fate of tetanus spores inoculated into guinea-pigs. Br. J. exp. Path 8:377
    [Google Scholar]
  6. Shoesmith J. G. 1964; The effect of heat and radiation on the viability and pathogenicity of Clostridium tetani. J. gen. Microbiol 35:vi
    [Google Scholar]
  7. Smith J. W. G. 1964; Penicillin in prevention of tetanus. Br. med. J 2:1293
    [Google Scholar]
  8. Smith J. W. G. 1966 In Studies in prophylaxis and treatment of tetanus M.D. Thesis University of Wales; p 269
    [Google Scholar]
  9. Smith J. W. G., MacIver A. G. 1969; Studies in experimental tetanus infection. J. med. Microbiol 2:385
    [Google Scholar]
  10. Suzuki J. B., Booth R., Benedik A., Grecz N. 1971; Pathogenesis of Clostridium botulinum, type A. Study of in vivo toxin release by implantation of diffusion chambers containing spores, vegetative cells and free toxin. Infect. Immun 3:659
    [Google Scholar]
  11. Suzuki J. B., Grecz N. 1972; Electron microscopy and leukocyte interaction with spores of Clostridium botulinum type A. Can. J. Microbiol 18:1651
    [Google Scholar]
  12. Vakil B. J., Aiyar S. N., Tulpule A. T., Mehta A. J., Tulpule T. H. 1965; A study of 2130 cases of tetanus with special reference to incidence, clinical types and prognosis. In Proceedings of the First International Conference on Tetanus, Bombay, 1963 edited by Patel J. C. Bombay: p 54
    [Google Scholar]
  13. Wright G. P. 1955; The neurotoxins of Clostridium botulinum and Clostridium tetani. Pharmac. Rev 7:413
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-7-4-497
Loading
/content/journal/jmm/10.1099/00222615-7-4-497
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error