%0 Journal Article %A Strizova, Vera %A Brown, Pamela K. %A Head, Beverley %A Reed, Sylvia E. %T The Advantages of Hela Cells For Isolation of Rhinoviruses %D 1974 %J Journal of Medical Microbiology, %V 7 %N 4 %P 433-438 %@ 1473-5644 %R https://doi.org/10.1099/00222615-7-4-433 %I Microbiology Society, %X SUMMARY A sensitive line of HeLa cells was found to be better than two lines of diploid human embryo-lung (HEL) cells, WI-38 and MRC-5, for the isolation of several serotypes of rhinovirus from experimentally-infected volunteers. Comparative infectivity titrations of three rhinoviruses which were either un-adapted or only partly adapted to tissue culture confirmed the greater sensitivity of the HeLa cells. The substitution of foetal calf serum for non-foetal bovine serum in the tissue culture medium slightly enhanced the sensitivity of the HEL cells to rhino viruses but had no such effect on HeLa cells; HeLa cells nevertheless remained more sensitive than HEL cells. The line of HeLa cells studied merits more general use for the isolation of rhino viruses from clinical specimens. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-7-4-433