1887

Abstract

SUMMARY

Fifty strains of Haemophilus influenzae and 30 strains of H. parainfluenzae were clearly differentiated by the requirement of H. influenzae strains for haemin on a proteose-peptone basal medium and with an inoculum of 1-20 cells. No evidence was obtained for a wide range in haemin requirements; 10 strains of H. influenzae examined in detail required 2-10 pg haemin per ml. The two species were dissimilar also in the amount of NAD required; the strains of H. influenzae grew well in the presence of NAD 0.2-1.0pg per ml, whereas most strains of H.parainfluenzae required 1.0-5.0pg per ml and three strains required NAD 25 pg per ml for optimal colony size. Nitrate was essential for the anaerobic growth of H. parainfluenzae but not of H. influenzae.

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1974-08-01
2022-11-30
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References

  1. Biberstein E. L., Gills M. 1961; Catalase activity of haemophilus species grown with graded amounts of hemin. J. Bad. 81:380
    [Google Scholar]
  2. Biberstein E. L., Spencer P. D. 1962; Oxidative metabolism of haemophilus species grown at different levels of hemin supplementation. J. Bad. 84:916
    [Google Scholar]
  3. Brumfitt W. 1959; Some growth requirements ofHaemophilus influenzae andHaemophilus pertussis. J. Path. Bad. 77:95
    [Google Scholar]
  4. Butler L. O. 1962; A defined medium forHaemophilus influenzae andHaemophilus parainfluenzae. J. gen. Microbiol. 27:51.
    [Google Scholar]
  5. Crawford J. J., Barden L., Kirkman J. B. 1969; Selective culture medium to survey the incidence of haemophilus species. Appl. Microbiol 18:646
    [Google Scholar]
  6. Cruickshank R. 1965; Medical microbiology. 11th ed London p 746
    [Google Scholar]
  7. Evans N. M., Smith D. D. 1972; The effect of the medium and source of growth factors on the satellitism test for haemophilus species. J. med. Microbiol. 5:509
    [Google Scholar]
  8. Fildes P. 1921; The nature of the effect of blood-pigment upon the growth ofB.influenzae. Br. J. exp. Path. 2:16
    [Google Scholar]
  9. Gilder H., Granick S. 1948; Studies on the hemophilus group of organisms. Quantitative aspects of growth on various porphin compounds. J. gen. Physiol. 31:103
    [Google Scholar]
  10. Herriott R. M., Meyer E. Y., Vogt M., Modan M. 1970; Defined medium for growth ofHaemophilus influenzae. J. Bact. 101:513
    [Google Scholar]
  11. Hovig B., Aandahl E. H. 1969; A selective method for the isolation ofHaemophilus in material from the respiratory tract. Acta. path, microbiol. scand. 77:676
    [Google Scholar]
  12. Klein M., Blazevic D. J. 1969; Differentiation of haemophilus species using satellite methods. Am. J. med. Technol. 35:695
    [Google Scholar]
  13. Koser S. A. 1968; Vitamin requirements of bacteria and yeasts. Springfield, Illinois, p p 282–285
    [Google Scholar]
  14. Lascelles J. 1962; Tetrapyrrole synthesis in microorganisms. In The Bacteria, a treatise on structure and function edited by Gunsalus I. C., Stainer R. Y. New York: vol Ill: Biosynthesis, p 335
    [Google Scholar]
  15. Lwoff A., Lwoff M. 1937; Studies on codehydrogenases. I. Nature of growth factor “ V ”.. Proc. R. Soc. B. 122:352
    [Google Scholar]
  16. Michaels R. H., Phillips D. M. 1972; Meningitis due to Haemophilus parainfluenzae. Am. J. Dis. Child. 124:788
    [Google Scholar]
  17. Parker R. H., Hoeprich P. D. 1962; Disk method for rapid identification of hemophilus species. Am. J. clin. Path. 37:319
    [Google Scholar]
  18. Pittman M. 1957 In Bergey’s Manual of Determinative Bacteriology. edited by Breed R. J., Murray E. D. G., Smith N. R. 7th ed Baltimore p 406
    [Google Scholar]
  19. Rivers T. M. 1922; Influenza-like bacilli. Growth of influenza-like bacilli on media containing only an autoclave-labile substance as an accessory food factor. Bull. Johns Hopkins Hosp. 33:429
    [Google Scholar]
  20. Smith W., Hale J. H., O’Callaghan C. H. 1953; Haem utilisation and nitrate reduction byHaemophilus influenzae. J. Path. Bact. 65:229
    [Google Scholar]
  21. Steers E., Foltz E. L., Graves B. S. 1959; An inocula replicating apparatus for routine testing of bacterial susceptibility to antibiotics. Antibiotics Chemother. 9:307
    [Google Scholar]
  22. Turk D. C., May J. R. 1967 Haemophilus influenzae, its clinical importance London: pp 11 and 116
    [Google Scholar]
  23. White D. C. 1963; Respiratory systems in the hemin-requiring haemophilus species. J. Bact. 85:84
    [Google Scholar]
  24. White D., Smith L. 1962; Haematin enzymes of Haemophilus parainfluenzae. J. biol. Chem. 237:1332
    [Google Scholar]
  25. Willis A. T. 1964; Anaerobic bacteriology in clinical medicine. 2nd ed London p 13
    [Google Scholar]
  26. Wolin H. L. 1963; Defined medium forHaemophilus influenzae typeb. J. Bact. 85:253
    [Google Scholar]
  27. Zinnemann K. 1960; Haemophilus influenzae and its pathogenicity. Ergebn. Mikrobiol. ImmunForsch. exp. Ther 33:307
    [Google Scholar]
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