1887

Abstract

SUMMARY

Eleven hundred and twenty-eight strains of anaerobic diphtheroids from human skin could be divided into two groups according to their colonial morphology on casein-yeast-lactate-glucose agar and their susceptibility to lysis by bacteriophages. Members of group I were sensitive and those of group I1 resistant to the action of phage. Biochemical tests confirmed this division among 200 strains, and permitted a subdivision of group I1 into a proteolytic and a non-proteolytic subgroup-IIA and IIB respectively. A simple scheme, based on colonial morphology, phage susceptibility, the production of indole, nitrate reduction, DNAase, gelatinase and caseinase formation, haemolysis, and the acidification of sucrose, sorbitol, and trehalose, could be used for the classification of these organisms.

Members of group I were most numerous on the skin of the scalp, forehead, and back, and in follicular material from the alae nasi. Group-IIA strains were found most often in the axilla, and group-IIB strains were occasionally present in small numbers at all sites.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-7-3-349
1974-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/7/3/medmicro-7-3-349.html?itemId=/content/journal/jmm/10.1099/00222615-7-3-349&mimeType=html&fmt=ahah

References

  1. Brzin B. 1964; Studies on the Corynebacterium acnes. Acta. path, microbiol. scand. 60:599
    [Google Scholar]
  2. Burtenshaw J. M. L. 1945; Self disinfection of the skin. Br. med. Bull. 3:161
    [Google Scholar]
  3. Cowan S. T., Steel K. J. 1965 Manual for the identification of medical bacteria London.:
    [Google Scholar]
  4. Evans C. A., Smith W. M., Johnston E. A., Giblett E. R. 1950; Bacterial flora of the normal human skin. J. invest. Derm. 15:305
    [Google Scholar]
  5. Holdeman L. V., Moore W. E. C. (editors) 1972 Anaerobe Laboratory Manual, VPI Anaerobe Laboratory; Blacksburg, Virginia, USA:
  6. Johnson J. L., Cummins C. S. 1972; Cell-wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibactera and strains ofArachnia propionica. J. Bact. 109:1047
    [Google Scholar]
  7. Lacey R. W. 1968; Antibacterial action of human skin. Br. J. exp. Path. 49:209
    [Google Scholar]
  8. Lacey R. W. 1969; Loss of the antibacterial action of skin after topical neomycin. Br. J. Derm. 81:435
    [Google Scholar]
  9. Marples M. J. 1965; The ecology of the human skin. Springfield
    [Google Scholar]
  10. Marples R. R. 1974; The microflora of the face and acne lesions. J. invest. Derm. 62:325
    [Google Scholar]
  11. Marples R. R., Downing D. T., Kligman A. M. 1971; Control of free fatty acids in human surface lipids byCorynebacterium acnes. J. invest. Derm. 56:127
    [Google Scholar]
  12. Marples R. R., Downing D. T., Kligman A. M. 1972; Influence ofPityrosporum species in the generation of free fatty acids in human surface lipids. J. invest. Derm. 58:155
    [Google Scholar]
  13. Marples R. R., McGinley K. J., Mills O. H. Jr 1973; Microbiology of comedones inacne vulgaris.. J. invest. Derm. 60:80
    [Google Scholar]
  14. Marples R. R., Leyden J. J., Stewart R. N., Mills O. H., Kligman A. M. 1974; The skin microflora in acne vulgaris.. J. invest. Derm. 62:37
    [Google Scholar]
  15. Martley F. G., Jayashankar S. R., Lawrence R. C. 1970; An improved agar medium for the detection of proteolytic organisms in total bacterial counts. J. appl. Bact 33:363
    [Google Scholar]
  16. Prévot A. R. 1966; Manual for the classification and determination of the anaerobic bacteria. (Translated by V. Fredette). Philadelphia
    [Google Scholar]
  17. Puhvel S. M. 1968; Characterization of Corynebacterium acnes. J. gen. Microbiol. 50:313
    [Google Scholar]
  18. Rasmussen E. G., Gibbons R. J., Socransky S. S. 1966; A taxonomic study of fifty gram positive anaerobic diphtheroids isolated from the oral cavity of man. Archs oral Biol 11:573
    [Google Scholar]
  19. Ray L. F., Kellum R. E. 1970; Corynebacterium acnes from human skin. Identifica tion by morphological, cultural, biochemical, serological and chromatographic methods. Archs Derm 101:36
    [Google Scholar]
  20. Reid J. D., Joya M. A. 1969; A study of the morphologic and biochemical character istics of certain anaerobic corynebacteria. Int. J. syst. Bact 19:273
    [Google Scholar]
  21. Rosebury T. 1962 Micro-organisms indigenous to man New York:
    [Google Scholar]
  22. Smith R. F., Bodily H. L. 1968; Identification ofCorynebacterium acnes. Hlth Lab. Sci. 5:100
    [Google Scholar]
  23. Strauss J. S., Kligman A. M. 1960; The pathologic dynamics of acne vulgaris. Archs. Derm 82:779
    [Google Scholar]
  24. Südmersen H. J., Thompson E. T. 1910; Cultivation and biological characters ofBacillus acnes. J. Path. Bact. 14:224
    [Google Scholar]
  25. Voss J. G. 1970; Differentiation of two groups ofCorynebacterium acnes. J. Bact. 101:392
    [Google Scholar]
  26. Williamson P., Kligman A. M. 1965; A new method for the quantitative investigation of cutaneous bacteria. J. invest. Derm. 45:498
    [Google Scholar]
  27. Zierdt C. H., Webster C., Rude W. S. 1968; Study of the anaerobic corynebacteria. Int. J. syst. Bact. 18:33
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-7-3-349
Loading
/content/journal/jmm/10.1099/00222615-7-3-349
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error