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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 7, Issue 2

The Inhibition of By Certain Agar and Peptone Preparations No Access

Abstract

SUMMARY

Proteose-peptone agar was defective for the culture of from small inocula when sterilising temperatures exceeded 116°C. All 23 strains tested grew poorly or not at all on the medium sterilised at temperatures of 121°C or more for 30 min. The inhibition was more marked when 5% CO was present during incubation. The inhibitory effect disappeared after the medium had been stored at 4°C for 7 days before inoculation, or could be abolished by the addition of a reducing agent such as sodium dithionite 100 g per ml.

The growth of may also be adversely affected by agar. Of three commercial agars tested, one batch of a particular brand was found to be inhibitory when used from a freshly opened bottle. Inhibition could not be demonstrated after the agar was washed or in the presence of sodium oleate (4 g per ml).

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1975
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1974-05-01
2025-07-15
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References

  1. Avery O. T. 1918; A selective medium for B. influenzae: oleate haemoglobin agar. J. Am. med. Ass 71:2050
     [Google Scholar]
  2. Balch H. H. 1942; Copper content of commercial peptones. J. Path. Bact 54:377
     [Google Scholar]
  3. Beale R. N., Croft D. 1964; The microdetermination of biological copper with oxalyldihydrazide. J. clin. Path 17:260
     [Google Scholar]
  4. Burnet F. M. 1925; Hydrogen peroxide and bacterial growth. Aust. J. exp. Biol. med. Sci 2:65
     [Google Scholar]
  5. Butler L. O. 1962; A defined medium for Haemophilus influenzae and Haemophilus parainfluenzae. J. gen. Microbiol 27:51
     [Google Scholar]
  6. Casman E. P. 1947; A noninfusion blood agar base for Neisseriae, Pneumococci and Streptococci. Am. J. clin. Path 17:281
     [Google Scholar]
  7. Cruickshank R. 1965; Medical microbiology, 11th ed.. London p 746
     [Google Scholar]
  8. Dubos R. 1930; The bacteriostatic action of certain components of commercial peptones as affected by conditions of oxidation and reduction. J. exp. Med 52:331
     [Google Scholar]
  9. Dubos R. J., Davis B. D. 1946; Factors affecting the growth of tubercle bacilli in liquid media. J. exp. Med 83:409
     [Google Scholar]
  10. Dukes C. D., Gardner H. L. 1961; Identification of Haemophilus vaginalis. J. Bact 81:277
     [Google Scholar]
  11. Evans N. M., Smith D. D. 1972; The effect of the medium and source of growth factors on the satellitism test for Haemophilus species. J. med. Microbiol 5:509
     [Google Scholar]
  12. Gilder H., Granick S. 1947; Studies on the Hemophilus group of organisms. Quantitative aspects of growth on various porphyrin compounds. J. gen. Physiol 31:103
     [Google Scholar]
  13. Gordon J., Mcleod J. W. 1926; Inhibition of bacterial growth by some amino-acids and its bearing on the use of tryptic digests as culture media. J. Path. Bact 29:13
     [Google Scholar]
  14. Hoagland C. L., Ward S. M., Gilder H., Shank R. E. 1942; Studies on the nutrition of Haemophilus influenzae. I. The relationship between the utilization of coenzyme and hemin and the reduction of nitrate. J. exp. Med 76:241
     [Google Scholar]
  15. Konowalchuk J., Hinton N. A., Reed G. B. 1954; Antibacterial action of a reaction product of cysteine and iron. 1. Development of the substance in media for Mycobacterium tuberculosis. Can. J. Microbiol 1:175
     [Google Scholar]
  16. Ley H. L., Mueller J. H. 1946; On the isolation from agar of an inhibitor for Neisseria gonorrhoeae. J. Bact 52:453
     [Google Scholar]
  17. Lwoff A., Lwoff M. 1937; Studies on codehydrogenases. I. Nature of growth factor “V ”. Proc. R. Soc. B 122:352
     [Google Scholar]
  18. Mazloum H. A., Rowley D. 1955; The growth requirements of Haemophilus pertussis on solid media. J. Path. Bact 70:439
     [Google Scholar]
  19. Nieman C. 1954; Influence of trace amounts of fatty acids on the growth of microorganisms. Bact. Rev 18:147
     [Google Scholar]
  20. O’Meara R. A. Q., Macsween J. C. 1936; The failure of Staphylococcus to grow from small inocula in routine laboratory media. J. Path. Bact 43:373
     [Google Scholar]
  21. Proom H., Woiwod A. J., Barnes J. M., Orbell W. G. 1950; A growth-inhibitory effect on Shigella dysenteriae which occurs with some batches of nutrient agar and is associated with the production of peroxide. J. gen. Microbiol 4:270
     [Google Scholar]
  22. Rowatt E. 1957; The growth of Bordetella pertussis: a review. J. gen. Microbiol 17:297
     [Google Scholar]
  23. Schuhardt V. T., Rode L. J., Oglesby G., Lankford C. E. 1950; The development of peptone toxicity for brucellae with aging and the correlation of this toxicity with the probable oxidation of cystine. J. Bact 60:655
     [Google Scholar]
  24. Society for General Microbiology 1956; Constituents of bacteriological culture media. Special report, edited by G. Sykes. Cambridge p 2
     [Google Scholar]
  25. Steers E., Foltz E. L., Graves B. S. 1959; An inocula replicating apparatus for routine testing of bacterial susceptibility to antibiotics. Antibiotics Chemother 9:307
     [Google Scholar]
  26. Traxler R. W., Lankford C. E. 1957; Observations on cystine degradation and bacteriotoxicity of peptones. Appl. Microbiol 5:70
     [Google Scholar]
  27. Woiwod A. J. 1954; The inhibition of bacterial growth by colloidal heavy-metal sulphides and by colloidal sulphur. J. gen. Microbiol 10:509
     [Google Scholar]
  28. WrighT H. D. 1929; The effect of certain factors upon the growth of the pneumococcus. J. Path. Bact 32:203
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-7-2-305
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The Inhibition of Haemophilus Influenzae By Certain Agar and Peptone Preparations
J. Med. Microbiol. 7, 305 (1974); https://doi.org/10.1099/00222615-7-2-305
/content/journal/jmm/10.1099/00222615-7-2-305
/content/journal/jmm/10.1099/00222615-7-2-305
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