1887

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Volume 7, Issue 2

Assay of Typhoid Vaccines in Mice Free

Abstract

SUMMARY

Protection of mice against challenge with virulent could be induced by intraperitoneal or subcutaneous vaccination with heat-phenol-or acetone-inactivated suspensions of strain Ty2 or of a Vi-producing strain of , or with Vi antigen prepared from either of these.

In all cases, protection rose to a maximum at about 4 days after vaccination. By day 18 this had fallen to the level at day 2.

The rise and fall of Vi agglutinins in mice vaccinated either intraperitoneally or subcutaneously with heat-phenol- or with acetone-killed suspensions of Ty2 or paralleled closely the rise and fall in protection, whereas the rise of O agglutinins corresponded with the decline in protection.

It was concluded that the potency tests for typhoid vaccines according to procedures SCM and IPM (WHO type-N procedures) in mice are chiefly a measure of the response to Vi antigen and consequently not a measure of factors responsible for eliciting immunity in man.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1975
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1974-05-01
2024-04-19
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References

  1. Department of Health, Education and Welfare, USA 1969; Additional standards; typhoid vaccine and limits of potency. Federal Register, USA 34:8914
     [Google Scholar]
  2. Esposito V. M., Feeley J. C. 1970; Isolation of Vi antigen from Escherichia coli by a new method. Infect. Immun 1:69
     [Google Scholar]
  3. Hejfets L. B., Levina L. A., Bentsianova T. G., Salmin L. V. 1968; The protective activity of various antigen complexes of typhoid and paratyphoid B vaccines: controlled field trials and laboratory studies. Proc. 11th Int. Congr. Microb. Standard., Milan In Prog, immunobiol. Standard, published by Karger; Basel: vol 4: p. 561, 1970
    [Google Scholar]
  4. Joó I., Pusztai Z., Juhasz V. P. 1968; Mouse-protective ability of the International Reference Preparations of typhoid vaccine. Z. ImmunForsch. exp. Ther 135:365
     [Google Scholar]
  5. Landy M. 1957; Studies on Vi antigen. VII. Characteristics of the immune response in the mouse. Am. J. Hyg 65:81
     [Google Scholar]
  6. Marmion D. E., Naylor G. R. E., Stewart I. O. 1953; Second attacks of typhoid fever. J. Hyg., Camb 51:260
     [Google Scholar]
  7. Pittman M., Bohner H. J. 1966; Laboratory assays of different types of field trial typhoid vaccines and relationship to efficacy in man. J. Bact 91:1713
     [Google Scholar]
  8. Sterne M. 1958; The growth of Brucella abortus strain 19 in aerated dialysed media. J. gen. Microbiol 18:747
     [Google Scholar]
  9. Sterne M., Trim G. 1968; The effects of non-specific agents and route of administration on the biological assay of Salmonella typhi vaccines. Intern. Symp. Enterobact. Vaccines, Berne In Symp. Series Immunobiol. Standard., published by Karger; Basel: vol 15: p. 93, 1971
    [Google Scholar]
  10. Sterne M., Trim G. 1970; Enhancement of the potency of typhoid vaccines with calcium alginate. J. med. Microbiol 3:649
     [Google Scholar]
  11. Sterne M., Trim G. 1971; The significance of protection tests in mice for evaluating typhoid vaccines. Proc. 12th Int. Congr. Microb. Standard., Annecy In Prog. Immunobiol. Standard., published by Karger; Basel: vol 5: p. 382, 1972
    [Google Scholar]
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Assay of Typhoid Vaccines in Mice
J. Med. Microbiol. 7, 197 (1974); https://doi.org/10.1099/00222615-7-2-197
/content/journal/jmm/10.1099/00222615-7-2-197
/content/journal/jmm/10.1099/00222615-7-2-197
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