RT Journal Article SR Electronic(1) A1 Hughes, J. H. A1 Thomas, D. C. A1 Hamparian, V. V. A1 Somerson, N. L.YR 1974 T1 Characterisation of Mycoplasma Pneumoniae Growth Factors In Bovine Serum Fraction JF Journal of Medical Microbiology, VO 7 IS 1 SP 35 OP 40 DO https://doi.org/10.1099/00222615-7-1-35 PB Microbiology Society, SN 1473-5644, AB SUMMARY Commercially available batches of bovine serum fraction (BSF) vary in their ability to promote the growth of Mycoplasma pneumoniae on glass. Poly-acrylamide gel electrophoresis shows the presence in BSF of three major components, which can also be separated by Sephadex chromatography. The slow moving component (SMC) in the gel appears to be the factor responsible for the adherence of M. pneumoniae to glass and its growth on that site; batches of BSF containing 6.15% of SMC possess high activity, those with less than 5% have low activity. Polyacrylamide gel electrophoresis is a simple and precise technique for determining growth-promoting activity of BSF., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-7-1-35