1887

Abstract

SUMMARY

Commercially available batches of bovine serum fraction (BSF) vary in their ability to promote the growth of on glass. Poly-acrylamide gel electrophoresis shows the presence in BSF of three major components, which can also be separated by Sephadex chromatography. The slow moving component (SMC) in the gel appears to be the factor responsible for the adherence of to glass and its growth on that site; batches of BSF containing 6.15% of SMC possess high activity, those with less than 5% have low activity. Polyacrylamide gel electrophoresis is a simple and precise technique for determining growth-promoting activity of BSF.

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/content/journal/jmm/10.1099/00222615-7-1-35
1974-02-01
2022-08-13
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