A simple method is described for the assay of gentamicin and kanamycin in human serum. It is a modification of the monosaccharide hydrolysis method of Faine and Knight (1968) and will estimate low concentrations of antibiotic accurately within 3 hours. The method gives results similar to those obtained by a double-row tube-dilution growth inhibition technique, is unaffected by the presence of penicillins or cephalosporins, and requires only standard laboratory equipment. Because the method gives readily reproducible results and is easy to perform, it should commend itself to all laboratories dealing with patients receiving aminoglycoside therapy.


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  1. Bennett J. V., Brodie J. L., Benner E. J., Kirby W. M. M. 1966; Simplified, accurate method of antibiotic assay of clinical specimens. Appl. Microbiol 14:170
    [Google Scholar]
  2. Dutton A. A. C., Elmes P. L. 1959; Vancomycin: report on treatment of patients with severe staphylococcal infections. B. med. J. 1:1144
    [Google Scholar]
  3. Faine S., Knight D. C. 1968; Rapid microbiological assay of antibiotic in blood and other body fluids . Lancet 2:375
    [Google Scholar]
  4. Garrod L. P., O’Grady F. 1971; Antibiotic and chemotherapy. 3rd ed London
    [Google Scholar]
  5. Muchison D. A., Spicer C. C. 1949; A method of estimating streptomycin in serum and other body fluids by diffusion through agar enclosed in glass tubes. J. gen. Microbiol 3:184
    [Google Scholar]
  6. Noone P., Pattison J. R., Samson D. 1971; Simple rapid method for assay of aminoglycoside antibiotics. Lancet 2:16
    [Google Scholar]

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