1887

Abstract

SUMMARY

Methods are described for the dialysis culture and subsequent concentration and purification of enterotoxin, or its formolised toxoid (anatoxin), by precipitation with zinc acetate. The techniques are simple and rapid, and are likely to be suitable for research and large-scale production. An 11-fold increase in specific activity, a 67 per cent. yield, and a 40-fold concentration were achieved in a single precipitation step. Immuno-electrophoretic data indicate that the purified toxin may be a complex of two different antigenic components.

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/content/journal/jmm/10.1099/00222615-6-4-499
1973-11-01
2022-10-03
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