1887

Journal of Medical Microbiology logo

Volume 6, Issue 3

Bactericidal Synergism of Novobiocin and Tetracycline Against Free

Abstract

SUMMARY

On the assumption that bactericidal action of drugs is probably required for the effective treatment of melioidosis, in-vitro experiments were performed with five chemotherapeutic agents and six strains from clinical sources. Chloramphenicol, kanamycin, novobiocin and tetracycline, alone and in various combinations, were tested for bactericidal activity against reasonably heavy inocula by means of a tube-dilution technique with subculture for viable counts. Results are displayed by Loewe’s method of isobolograms in a simplified form. The novobiocin-tetracycline combination produced a kill of 99.4 to 99.8 per cent. with five of the six strains at concentrations at which neither drug had any effect when used alone (2.5 to 10 μg per ml of novobiocin and 2 to 5 μg per ml of tetracycline). The synergistic effect of this mixture was demonstrable with as little as 0.2 μg per ml of novobiocin, or 0.003 of the concentration needed for its comparable independent effect; in clinical use the latter could be achieved systemically only with unusually high dosage. No bactericidal effect was found with one strain.

Sulphamylon showed a strong bactericidal effect at concentrations that might be achieved locally by topical applications to wounds and burns.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1973
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-6-3-293
1973-08-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/6/3/medmicro-6-3-293.html?itemId=/content/journal/jmm/10.1099/00222615-6-3-293&mimeType=html&fmt=ahah

References

  1. Alain M., Saint-Etienne J., Reynes V. 1949; La méioïdose. Considerations étiologiques, cliniques et pathog£niques k propos de 28 cas. Med. trop 9:119
     [Google Scholar]
  2. Borchardt K. A., Stansifer P., Albano P. M. 1966; Osteomyelitis due to Pseudomonas pseudomallei. J. Am. Med. Ass 196:660
     [Google Scholar]
  3. Bromwich A. F., Hargrave J. C. 1969; Melioidosis in an Australian aboriginal with lepromatous leprosy. Med. J. Aust 1:638
     [Google Scholar]
  4. Brundage W. G., Thuss C. J., Walden D. C. 1968; Four fatal cases of melioidosis in U,. S. soldiers in Vietnam. Bacteriologic and pathologic characteristics. Am. J. Trop. Med. Hyg 17:183
     [Google Scholar]
  5. Brygoo E. R. 1953; Action du chloramphenicol sur le bacille de Whitmore. Bull. Soc. Path, exot 46:169
     [Google Scholar]
  6. Byrd R. B., Puritz E. M. 1970; Melioidosis. Postgrad. Med 47:144
     [Google Scholar]
  7. Caubet J. 1953; Mélioïdose. In Rapport sur le fonctionnement technique de l’lnstitut Pasteur de Saigon en 1952. Saigon p 34
     [Google Scholar]
  8. Chambon L. 1955a; Isolement du bacille de Whitmore k partir du milieu exterieur. Annls Inst. Pasteur 89:229
     [Google Scholar]
  9. Chambon L. 1955b; Resistance du bacille de Whitmore acquise in vitro et in vivo a regard du chloramphenicol, de l’aureomycine et de la terramycine. Annls Inst. Pasteur 88:315
     [Google Scholar]
  10. Chambon L., De Lajudie P., Fournier J. 1954; Etude de la sensibilité du bacille de Whitmore aux antibiotiques in vitro et chez les malades atteints de m & ioldose. Bull. Soc. Path, exot 47:139
     [Google Scholar]
  11. Cooper E. B. 1967; Melioidosis. J. Am. Med. Ass 200:452
     [Google Scholar]
  12. Cowan S. T., Steel K. J. 1965; Manual for the identification of medical bacteria. Cambridge pp 71, 81
     [Google Scholar]
  13. Duong-Hong-Mo, Tran-Hiep-Cuong. 1967; A typical case of melioidosis in South Vietnam. Milit. Med 132:98
     [Google Scholar]
  14. Eickhoff T. C., Bennett J. V., Hayes Peggy S., Feeley J. 1970; Pseudomonas pseudomallei: susceptibility to chemotherapeutic agents. J. Infect. Dis 121:95
     [Google Scholar]
  15. Fisher M. W., Hillegas A. B., Nazeeri Patsy. 1971; Susceptibility in vitro and in vivo of Pseudomonas pseudomallei to rifampin and tetracyclines. Appl. Microbiol 22:13
     [Google Scholar]
  16. Flemma R. G., Divincenti F. C., Dotin L. N., Pruitt B. A. 1969; Pulmonary melioidosis: a diagnostic dilemma and increasing threat. Ann. Thorac. Surg 7:491
     [Google Scholar]
  17. Foley F. D., Greenawald K. A., Nash G., Pruitt B. A. 1969; Pathology of pseudomonas infection. Tex. Med 65:36
     [Google Scholar]
  18. Fournier J., Chambon L. 1958; La mëioïdose et le bacille de Whitmore. Paris
     [Google Scholar]
  19. Garrod L. P., O’Grady F. 1968; Antibiotic and chemotherapy. 2nd ed Edinburgh and London pp 40, 300
     [Google Scholar]
  20. Green R., Mankikar D. S. 1949; Afebrile cases of melioidosis. Brit. Med. J 1:308
     [Google Scholar]
  21. Green R. N., Tuffnell P. G. 1968; Laboratory acquired melioidosis. Am. J. Med 44:599
     [Google Scholar]
  22. Greenawald K. A., Nash G., Foley F. D. 1969; Acute systemic melioidosis. Autopsy findings in four patients. Am. J. Clin. Path 52:188
     [Google Scholar]
  23. Guillermand J., Barrié J., Morilleau R., Couderc P., Ly Baysy. 1964; Mè1ioïdose pulmonaire chronique. Observation anatomoclinique. Poumon coeur 20:269
     [Google Scholar]
  24. Haynes W. C., Burkholder W. H. 1957 In Bergey’s manual of determinative bacteriology. 7th ed., edited by Breed R. S., Murray E. D. G., Smith N. R. Baltimore p 100
     [Google Scholar]
  25. Hewlett P. S. 1969; Measurement of the potencies of drug mixtures. Biometrics 25:477
     [Google Scholar]
  26. Kishimoto R. A., Brown G. L., Blair E. B., Wenkheimer Dorothy. 1971; Melioidosis: serologic studies on U,. S. personnel returning from Southeast Asia. Milit. Med 136:694
     [Google Scholar]
  27. Lindberg R. B., Moncrief J. A., Switzer W. E., Order S. E., Mills W. 1965; The successful control of burn wound sepsis. J. Trauma 5:601
     [Google Scholar]
  28. Loewe S. 1953; The problem of synergism and antagonism of combined drugs. Arzneimittel-Forsch 3:285
     [Google Scholar]
  29. Mcdowell F., Varney P. L. 1947; Melioidosis. Report of first case from the western hemisphere. J. Am. Med. Ass 134:361
     [Google Scholar]
  30. Maegraith B. G., Leithead C. S. 1964; Melioidosis: a case report. Lancet 1:862
     [Google Scholar]
  31. Magee H. R., Mitchell R. M., Fitzwater J. J., Christie D. J. S., Rao A. 1967; Melioidosis. Med. J. Aust 1:1180
     [Google Scholar]
  32. Moncrief J. A., Lindberg R. B., Switzer W. E., Pruitt B. A. 1966; The use of a topical sulfonamide in the control of bum wound sepsis. J. Trauma 6:407
     [Google Scholar]
  33. Morrison I. M. 1970; Chronic melioidosis. Proc. Roy. Soc. Med 63:289
     [Google Scholar]
  34. Patterson M. C., Darling C. L., Blumenthal J. B. 1967; Acute melioidosis in a soldier home from South Vietnam. J. Am. Med. Ass 200:447
     [Google Scholar]
  35. Prevatt A. L., Hunt J. S. 1957; Chronic melioidosis: review of literature and report of a case, with a note on visual disturbance due to chloramphenicol. Am. J. Med 23:810
     [Google Scholar]
  36. Rowlands J. B., Curtis P. G. 1965; A case of melioidosis in Papua and New Guinea. Med. J. Aust 2:494
     [Google Scholar]
  37. Salisbury W. A., Likos J. J. 1970; Pseudomonas pseudomallei in a case of chronic melioidosis. Am. J. Clin. Path 54:602
     [Google Scholar]
  38. Spotnitz M., Rudnitsky J., Rambaud J. J. 1967; Melioidosis pneumonitis. J. Am. Med. Ass 202:950
     [Google Scholar]
  39. Stanton A. T., Fletcher W. 1932; Melioidosis. Studies from the Institute for Medical Research, Federated Malay States. no. 21 London
     [Google Scholar]
  40. Tarlow M. J. 1971; Melioidosis and chronic granulomatous disease. Proc. Roy. Soc. Med 64:19
     [Google Scholar]
  41. Thin R. N., Brown M., Stewart J. B., Garrett C. J. 1970; Melioidosis: a report of ten cases. Quart. J. Med 39:115
     [Google Scholar]
  42. Weber D. R., Douglass L. E., Brundage W. G., Stallkamp T. C. 1969; Acute varieties of melioidosis occurring in U,. S. soldiers in Vietnam. Am. J. Med 46:234
     [Google Scholar]
  43. Zimmerman J. E. 1970; Acute septicemic melioidosis. J. Am. Med. Ass 213:2266
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-6-3-293
Loading
Bactericidal Synergism of Novobiocin and Tetracycline Against Pseudomonas Pseudomallei
J. Med. Microbiol. 6, 293 (1973); https://doi.org/10.1099/00222615-6-3-293
/content/journal/jmm/10.1099/00222615-6-3-293
/content/journal/jmm/10.1099/00222615-6-3-293
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error