1887

Abstract

SUMMARY

Specimens from 466 patients with acute respiratory-tract illnesses that failed to yield a causative agent by the standard methods of virus isolation were examined in organ cultures. Seventy-three viruses (15.7 per cent) were isolated. Most of them were rhinoviruses (81.0 per cent.) but seven coronaviruses, four of which were 229E-like, were also isolated. The majority of the rhinoviruses were detected in the initial organ-culture fluids, but several blind passages were needed for the organ-culture strains of coronaviruses. Although laborious, organ culture must remain an integral part of any detailed study of acute infections of the respiratory tract.

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1973-05-01
2024-04-16
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References

  1. Bradburne A. F., Tyrrell D. A. J. 1969; The propagation of “ coronaviruses ” in tissue-culture. Arch. ges. Virusforsch 28:133
    [Google Scholar]
  2. Butler M., Ellaway Wendy J., Hall T. 1972; Comparative studies on the infectivity of avian respiratory viruses for eggs, cell cultures and tracheal explants. J. Comp. Path. Ther 82:327
    [Google Scholar]
  3. Davis E. V., Bolin V. S. 1960; Continuous cultivation of isogenous cell lines from the human embryo. Fed. Proc 19:386
    [Google Scholar]
  4. Hamre Dorothy, Procknow J. J. 1966; A new virus isolated from the human respiratory tract. Proc. Soc. Exp. Biol. Med 121:190
    [Google Scholar]
  5. Higgins P. G. 1966; The isolation of viruses from acute respiratory infections. V. The use of organ cultures of human embryonic nasal and tracheal ciliated epithelium. Mon. Bull. Minist. Hlth 25:283
    [Google Scholar]
  6. Higgins P. G., Boston D. G., Ellis Eileen M. 1964; The isolation of viruses from acute respiratory infections. II. A study of the isolations made from cases occurring in a general practice in (1963). Mon. Bull. Min. Hlth 23:93
    [Google Scholar]
  7. Higgins P. G., Ellis E. M. 1972; The isolation of influenza viruses. J. Clin. Path 25:521
    [Google Scholar]
  8. Higgins P. G., Ellis Eileen M, Boston Doreen G. 1963; The isolation of viruses from acute respiratory infections. A study of the isolations made from cases occurring in a general practice in (1962). MonBull. Min. Hlth 22:71
    [Google Scholar]
  9. Higgins P. G., Ellis Eileen M., Woolley D. A. 1969; A comparative study of standard methods and organ culture for the isolation of respiratory viruses. J. Med. Microbiol 2:109
    [Google Scholar]
  10. Higgins P. G., Ellis Eileen M., Woolley D. A, Cassidy P. F. 1970; Viruses associated with acute respiratory infections in Royal Air Force personnel. J. Hyg Camb 68:647
    [Google Scholar]
  11. Hoorn B. 1966; Organ cultures of the ciliated epithelium for the study of respiratory viruses. Acta path, microbiol. scand 66: Suppl 183
    [Google Scholar]
  12. Kapikian A. Z., James H. D. Jr, Kelly Sara J., Dees Jane H., Turner H. C., McIntosh K., Kim H. W., Parrott R. H., Vincent M. M., Chanock R. M. 1969; Isolation from man of “ avian infectious bronchitis virus-like ” viruses (coronaviruses) similar to 229E virus, with some epidemiological observations. J. Infect. Dis 119:282
    [Google Scholar]
  13. McIntosh K., Dees Jane H., Becker W. B., Kapikian A. Z., Chanock R. M. 1967; Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natn. Acad. Sci. U.S.A 57:933
    [Google Scholar]
  14. Mostow S. R., Tyrrell D. A. J. 1972; Detection of attenuation of recombinant influenza viruses in vitro. Lancet 2:116
    [Google Scholar]
  15. Roome A. P. C. H., Dickinson Valerie, Caul E. O. 1971; Simplified organ cultures of human embryo trachea in the diagnosis of viral respiratory disease of children. J. Clin. Path 24:487
    [Google Scholar]
  16. Tyrrell D. A. J., Blamire C. J. 1967; Improvements in a method of growing respiratory viruses in organ cultures. Br. J. Exp. Path 48:217
    [Google Scholar]
  17. Tyrrell D. A. J., Bynoe M. L. 1965; Cultivation of a novel type of common-cold virus in organ cultures. Br. med. J 1:1467
    [Google Scholar]
  18. Tyrrell D. A. J., Bynoe M. L. 1966; Cultivation of viruses from a high proportion of patients with colds. Lancet 1:76
    [Google Scholar]
  19. Tyrrell D. A. J., Bynoe M. L., Hoorn B. 1968; Cultivation of “ difficult ” viruses from patients with common colds. Br. med. J 1:606
    [Google Scholar]
  20. Wigand R., Sabin A. B. 1961; Properties of ECHO types 22, 23 and 24 viruses. Arch. ges. Virusforsch 11:224
    [Google Scholar]
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