Joint Trimethoprim and Sulphamethoxazole Resistance in Bacteria Infected with R Factors Free

Abstract

SUMMARY

Gram-negative bacteria, harbouring R factors that confer resistance to sulphonamides, mutate to trimethoprim resistance at an appreciable rate and are then able to grow in the presence of both trimethoprim and a sulphonamide.

All trimethoprim-resistant organisms isolated in this study were thymine requiring, and it is suggested that the frequency of isolation of organisms resistant to trimethoprim, or combinations of trimethoprim and a sulphonamide, may be increased if adequate thymine or thymidine is included in the media used for their isolation.

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/content/journal/jmm/10.1099/00222615-6-1-13
1973-02-01
2024-03-28
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References

  1. Bertino J. B., Stacey K. A. 1966; A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli . Biochem. J. 101:32C
    [Google Scholar]
  2. Bushby S. R. M. 1969; Combined antibacterial action in vitro of trimethoprim and sulphonamides. Post-grad. Med. J. 45: Suppl 10
    [Google Scholar]
  3. Crawford L. V. 1958; Thymine metabolism in strains of Escherichia coli . Biochim. biophys. Acta 30:428
    [Google Scholar]
  4. Darrell J. H., Garrod L. P., Waterworth L., Pamela M. 1968; Trimethoprim: laboratory and clinical studies. J. Clin. Path. 21:202
    [Google Scholar]
  5. Datta, Naomi, Kontomichalou, Polyxeni. 1965; Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae. Nature, Lond 208:239
    [Google Scholar]
  6. Davis B. D., Mingioli, Elizabeth. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12. J. Bact. 60:17
    [Google Scholar]
  7. Garrod L. P. 1971; Trimethoprim: its possible place in antibacterial therapy. Drugs 1:3
    [Google Scholar]
  8. Hitchings G. H. 1969; Specific differences among dihydrofolate reductases as a basis for chemotherapy. Post-grad. Med. J. 45: Suppl 7
    [Google Scholar]
  9. Kammen H. O. 1967; Thymine metabolism in Escherichia coli. I. Factors involved in utilization of exogenous thymine. Biochim. biophys. Acta 134:301
    [Google Scholar]
  10. Koch, Audrey E., Burchall J. J. 1971; Reversal of the antimicrobial activity of trimethoprim by thymidine in commercially prepared media. Appl. Microbiol. 22:812
    [Google Scholar]
  11. Meynell, Elinor, Datta, Naomi. 1966; The relation of resistance transfer factors to the F-factor (sex-factor) of Escherichia coli K12. Genet. Res. 7:134
    [Google Scholar]
  12. Pinney R. J., Smith J. T. 1971; R-factor elimination by thymine starvation. Genet. Res. 18:173
    [Google Scholar]
  13. Smith J. T. 1969; R-factor gene expression in Gram-negative bacteria. J. Gen. Microbiol. 55:109
    [Google Scholar]
  14. Stacey K. A., Simson, Eva. 1965; Improved method for the isolation of thymine-requiring mutants of Escherichia coli . J. Bact. 90:554
    [Google Scholar]
  15. Watanabe T., Fukasawa T. 1961; Episome-mediated transfer of drug resistance in Enterobacteriaceae. I. Transfer of resistance factors by conjugation. J. Bact. 81:669
    [Google Scholar]
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