1887

Abstract

SUMMARY

Human embryonic tracheal organ cultures have been frozen to — 196°C and thawed without any apparent loss of ciliary activity or susceptibility to the growth of human influenza virus. Optimal preservation was obtained by freezing in the presence of 25 per cent. dimethylsulphoxide at a cooling rate of 0.3°C per min. This extremely simple technique facilitates the storage of tracheal organ cultures for experimental use.

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/content/journal/jmm/10.1099/00222615-6-1-123
1973-02-01
2022-01-22
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