Salmonellae present in or artificially inoculated into sewage were readily isolated by plating selenite F enrichment cultures on deoxycholate citrate agar provided the plates were incubated anaerobically in the presence of about 10 per cent. (v/v) carbon dioxide. Under these conditions, salmonellae gave distinctive jet-black colonies, pseudomonads were suppressed and the growth of was markedly inhibited. A modified procedure was required for satisfactory recovery of , notably the use of double-strength selenite medium and the omission of lactose from the deoxycholate citrate agar.


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