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Volume 5, Issue 4

Studies On Staphylokinase Free

Abstract

SUMMARY

Staphylokinase in the supernatant of cultures of was concentrated and then purified twice successively by isoelectric focusing. The total activity of staphylokinase was increased about 100 per cent. after the first isoelectric focusing. The cause of this was investigated. It was also shown by means of this separation technique that staphylokinase is a heterogenous protein, with isoelectric points at H 5.8 (component A), 6.2 (component B) and 6.8 (component C). The degree of purification was calculated.

Some properties of the components were studied. The effects of the following metal ions: Ca, Mg, Mn, Cu, Pb, Zn, Ag and Co; and of EDTA and cysteine, were examined. No significant differences in these respects between the three components were observed. The H for optimal activity also seemed to be the same for the main components.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1972
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1972-11-01
2024-04-24
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References

  1. Davidson Flora M. 1960; The Activation Of Plasminogen By Staphylokinase: Comparison With Streptokinase. Biochem. J 76:56
     [Google Scholar]
  2. Gerheim E. B. 1948; Staphylococcal Coagulation And Fibrinolysis. Nature, Lond 162:732
     [Google Scholar]
  3. Glanville Kitty L. A. 1963; A simple method of purifying staphylokinase. Biochem. J 88:11
     [Google Scholar]
  4. Lack C. H. 1948; Staphylokinase: an activator of plasma protease. Nature, Lond 161:559
     [Google Scholar]
  5. Lack C. H., Glanville Kitty L. A. 1970 Staphylokinase. In Methods in enzy-mology edited by Colowick S. P., Kaplan N. O. New York: vol. 19 p. 706
     [Google Scholar]
  6. Lewis Jessica H., Ferguson J. H. 1951; A proteolytic enzyme system of the blood. III. Activation of dog serum profibrinolysin by staphlokinase. Amer. J. Physiol 166:594
     [Google Scholar]
  7. Lewis Jessica H., Kerber C. W., Wilson J. H. 1964; Effects of fibrinolytic agents and heparin on intravascular clot lysis. Amer. J. Physiol 207:1044
     [Google Scholar]
  8. Lowry O. H., Rosebrough N. J., Farr A. L., Randall Rose J. 1951; Protein measurement with the Folin phenol reagent. J. Biol. Chem 193:265
     [Google Scholar]
  9. Soru E., Sternberg M., Istrati M. 1959; Contribution à L’étude Chimique De La Staphylokynase. Naturwissenschaften 46:496
     [Google Scholar]
  10. Sgouris J. T., Inman J. K., Mccall K. B., Hyndman L. A., Anderson H. D. 1960; The Preparation Of Human Fibrinolysin (Plasmin). Vox Sang 5:357
     [Google Scholar]
  11. Wadström T. 1967; Studies on extracellular proteins from Staphylococcus aureus. II. Separation of deoxyribonucleases by isoelectric focusing. Purification and properties of the enzymes. Biochim. biophys. Acta 147:441
     [Google Scholar]
  12. Wadström T. 1968; Studies on extracellular proteins from Staphylococcus aureus. IV. Separation of α-toxin by isoelectric focusing. Biochim. biophys. Acta 168:228
     [Google Scholar]
  13. Vesterberg O., Wadström T., Vesterberg K., Svensson H., Malmgren B. 1967; Studies on extracellular proteins from Staphylococcus aureus I. Separation and characterization of enzymes and toxins by isoelectric focusing. Biochim. biophys. Acta 133:435
     [Google Scholar]
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Studies On Staphylokinase
J. Med. Microbiol. 5, 441 (1972); https://doi.org/10.1099/00222615-5-4-441
/content/journal/jmm/10.1099/00222615-5-4-441
/content/journal/jmm/10.1099/00222615-5-4-441
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