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A heat-stable enterotoxin from a strain of Escherichia coli enteropathogenic for calves was subjected to dialysis and ultrafiltration, and the resulting fractions were tested in Thiry-Vella loops in calves.
The active material was dialysable, and could be demonstrated in the dialysate. After 7 days’ dialysis, the activity remaining in the dialysand was not significant.
Ultrafiltration suggested that the molecular weight of most of the active material was between 1000 and 10,000, although a small amount appeared to have a molecular weight less than 1000.
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