1887

Abstract

Summary

The pathogenesis of infection was studied in normal mice and in thymectomised, X-irradiated, anti-lymphocyte-treated mice. The immunosuppressed mice were more readily infected by the intranasal route than were normal mice, although the mycoplasmas multiplied to only a slightly greater extent in the lungs of the deficient animals. Spread of mycoplasmas from the lungs to other organs, such as the brain, occurred more frequently in the immunologically deficient mice. Cell-mediated immunity appeared to be important in the development of the pulmonary lesions. Thus, the lungs of normal mice showed marked peribronchiolar and perivascular cuffing by lymphocytes, but this was much less prominent in the immunosuppressed mice and macrophages were also fewer in number.

Immunofluorescence studies provided further evidence for the important role of cell-mediated immune mechanisms in infections in mice. The way in which such mechanisms may operate is discussed.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-5-3-327
1972-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/5/3/medmicro-5-3-327.html?itemId=/content/journal/jmm/10.1099/00222615-5-3-327&mimeType=html&fmt=ahah

References

  1. Allison A. C. 1970 On the role of macrophages in some pathological processes. In Mononuclear phagocytes edited by van Furth R. Oxford and Edinburgh: p. 422
    [Google Scholar]
  2. Allison A. C. 1971a Immunity against viruses. In Scientific basis of medicine London: p. 49
    [Google Scholar]
  3. Allison A. C. 1971b; The role of membranes in the replication of animal viruses. Int. Rev. Exp. Path 10:181
    [Google Scholar]
  4. Biberfeld G., Sterner G. 1969; Antibodies against Mycoplasma pneumoniae in bronchial secretions. Acta path, microbiol. scand 76:646
    [Google Scholar]
  5. Biberfeld G., Sterner G. 1971; Antibodies in bronchial secretions following natural infection with Mycoplasma pneumoniae. Acta path, microbiol. scand 79B:599
    [Google Scholar]
  6. Chanock R. M., Hayflick L., Barile M. F. 1962; Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc. Natn. Acad. Sci. U.S.A 48:41
    [Google Scholar]
  7. Clyde W. A. Jr 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. J. Immun 92:958
    [Google Scholar]
  8. Collier A. M., Clyde W. A. Jr 1971; Relationships between Mycoplasma pneumoniae and human respiratory epithelium. Infect. Immun 3:694
    [Google Scholar]
  9. Collier A. M., Clyde W. A. Jr, Denny F. W. 1969; Biologic effects of Mycoplasma pneumoniae and other mycoplasmas from man on hamster tracheal organ culture. Proc. Soc. Exp. Biol. Med 132:1153
    [Google Scholar]
  10. Dajani A. S., Clyde W. A. Jr, Denny F. W. 1965; Experimental infection with Mycoplasma pneumoniae (Eaton’s agent). J. Exp. Med 121:1071
    [Google Scholar]
  11. Fernald G. W. 1969; Immunologic aspects of experimental Mycoplasma pneumoniae infection. J. Infect. Dis 119:255
    [Google Scholar]
  12. Fernald G. W. 1970 Local immune mechanisms in respiratory infection due to Mycoplasma pneumoniae. In The secretory immunologic system edited by Dayton D. H. Jr others Washington, D. C.: p. 215
    [Google Scholar]
  13. Fernald G. W. 1971; Mycoplasma pneumoniae delayed hypersensitivity: skin tests in guinea pigs. Bact. Proc103
    [Google Scholar]
  14. Fernald G. W. 1972 Role of host response in Mycoplasma pneumoniae disease. In National Institute of Allergy and Infectious Diseases workshop on the Mycoplasmatales as agents of disease edited by Kenny G. J. Infect. Dis. in press
    [Google Scholar]
  15. Fernald G. W., Clyde W. A. Jr 1970; Protective effect of vaccines in experimental Mycoplasma pneumoniae disease. Infect. Immun 1:559
    [Google Scholar]
  16. Jones T. C., Hirsch J. G. 1971; The interaction in vitro of Mycoplasma pulmonis with mouse peritoneal macrophages and L-cells. J. Exp. Med 133:231
    [Google Scholar]
  17. Lemcke Ruth M. 1961; Association of PPLO infection and antibody response in rats and mice. J. Hyg., Camb 59:401
    [Google Scholar]
  18. Leventhal B. G., Smith C. B., Carbone P. P., Hersh E. M. 1969 Lymphocyte transformation in response to M. pneumoniae after experimental infection in man. In Proceedings of the Third Annual Leucocyte Culture Conference, 1967 edited by Rieke W. O. New York: p. 519
    [Google Scholar]
  19. Lipman R. P., Clyde W. A. Jr 1969; The interrelationship of virulence, cytadsorption, and peroxide formation in Mycoplasma pneumoniae. Proc. Soc. Exp. Biol. Med 131:1163
    [Google Scholar]
  20. Lutsky I. I., Organick A. B. 1966; Pneumonia due to mycoplasma in gnotobiotic mice. I. Pathogenicity of Mycoplasma pneumoniae, Mycoplasma salivarium, and Mycoplasma pulmonis for the lungs of conventional and gnotobiotic mice. J. Bact 92:1154
    [Google Scholar]
  21. Mackaness G. B. 1970a; Cellular immunity. In Mononuclear phagocytes edited by van Furth R. Oxford and Edinburgh: p. 461
    [Google Scholar]
  22. Mackaness G. B. 1970b; The monocyte in delayed-type hypersensitivity. In Mononuclear phagocytes edited by van Furth R. Oxford and Edinburgh: p. 478
    [Google Scholar]
  23. Manchee R. J., Taylor-Robinson D. 1969; Studies on the nature of receptors involved in attachment of tissue culture cells to mycoplasmas. Br. J. Exp. Path 50:66
    [Google Scholar]
  24. Mazzali Rosandra, Taylor-Robinson D. 1971; The behaviour of T-mycoplasmas in tissue culture. J. Med. Microbiol 4:125
    [Google Scholar]
  25. Nathanson N., Cole G. A. 1970; Immunosuppression and experimental virus infection of the nervous system. Adv. Virus Res 16:397
    [Google Scholar]
  26. Organick A. B., Lutsky I. I. 1968; Pneumonia due to mycoplasma in gnotobiotic mice. IV. Localization and identification of Mycoplasma pulmonis in the bronchi of infected gnotobiotic mice by immunofluorescence and by light microscopy. J. Bact 96:250
    [Google Scholar]
  27. Organick A. B., Siegesmund K. A., Lutsky I. I. 1966; Pneumonia due to mycoplasma in gnotobiotic mice. II. Localization of Mycoplasma pulmonis in the lungs of infected gnotobiotic mice by electron microscopy. J. Bact 92:1164
    [Google Scholar]
  28. Smith C. B., Chanock R. M., Friedewald W. T., Alford R. H. 1967; Mycoplasma pneumoniae infections in volunteers. Ann. N. Y. Acad. Sci 143:471
    [Google Scholar]
  29. Smith C. B., Friedewald W. T., Chanock R. M. 1967; Inactivated Mycoplasma pneumoniae vaccine. Evaluation in volunteers. J. Amer. Med. Assoc 199:353
    [Google Scholar]
  30. Sobeslavsky O., Prescott B., Chanock R. M. 1968; Adsorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. J. Bact 96:695
    [Google Scholar]
  31. Taylor-Robinson D., Denny F. W., Thompson G. W., Allison A. C., Märdh P.-A. 1972 Isolation of mycoplasmas from lungs by a perfusion technique. Med. Microbiol. Immun in press;
    [Google Scholar]
  32. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966; A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J. Hyg., Camb 64:91
    [Google Scholar]
  33. Zucker-Franklin Dorothea, Davidson M., Thomas L. 1966; The interaction of mycoplasmas with mammalian cells. I. HeLa cells, neutrophils, and eosinophils. J. Exp. Med 124:521
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-5-3-327
Loading
/content/journal/jmm/10.1099/00222615-5-3-327
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error