1887

Abstract

Summary

A simple method is described for the inactivation of contaminating bacteriophage in concentrated pyocin preparations prepared from induced lysates of . It is possible to destroy the plaque-forming ability of the bacteriophage by treatment with ultraviolet light and at the same time to preserve approximately 65 per cent. of the pyocin activity.

The effect of ultraviolet light on a concentrated bacteriophage preparation was studied, and it was shown that some residual inhibition activity remained. However, it is not likely that the lysogenic phage in concentrated pyocin preparations will be of a high enough titre to produce inhibition reactions.

It is now proposed to use pyocin extracts prepared in this way as typing reagents in a pyocin typing scheme based on the inhibition patterns produced when a number of different pyocins are spotted on to lawns of unknown test strains.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-5-3-305
1972-08-01
2024-04-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/5/3/medmicro-5-3-305.html?itemId=/content/journal/jmm/10.1099/00222615-5-3-305&mimeType=html&fmt=ahah

References

  1. Cohen S. S., Arbogast Rachel. 1950; Chemical studies in host-virus interactions. VIII. The mutual reactivation of T2r+ virus inactivated by ultraviolet light and the synthesis of desoxyribose nucleic acid. J. Exp. Med 91:637
    [Google Scholar]
  2. Darrell J. H., Wahba A. H. 1964; Pyocine-typing of hospital strains of Pseudomonas pyocyanea. J. Clin. Path 17:236
    [Google Scholar]
  3. Dulbecco R. 1950; Experiments on photoreactivation of bacteriophages inactivated with ultraviolet radiation. J. Bact 59:329
    [Google Scholar]
  4. III Farmer J. J., Herman L. G. 1969; Epidemiological fingerprinting of Pseudomonas aeruginosa by the production of and sensitivity to pyocin and bacteriophage. Appl. Microbiol 18:760
    [Google Scholar]
  5. Gillies R. R., Govan J. R. W. 1966; Typing of Pseudomonas pyocyanea by pyocine production. J. Path. Bact 91:339
    [Google Scholar]
  6. Gould J. C., McLeod J. W. 1960; A study of the use of agglutinating sera and phage lysis in the classification of strains of Pseudomonas aeruginosa. J. Path. Bact 79:295
    [Google Scholar]
  7. Harm W. 1961; Gene-controlled reactivation of ultraviolet-inactivated bacteriophage. J. Cell. Comp. Physiol 58:Suppl. 169
    [Google Scholar]
  8. Jacob F. 1954; Biosynthèse induite et mode d’action d’une pyocine, antibiotique de Pseudomonas pyocyanea. Annls. Inst. Pasteur, Paris 86:149
    [Google Scholar]
  9. Kageyama M. 1964; Studies of a pyocin. I. Physical and chemical properties. J. Biochem., Tokyo 55:49
    [Google Scholar]
  10. Kageyama M., Ikeda K., Egami F. 1964; Studies of a pyocin. III. Biological properties of the pyocin. J. Biochem., Tokyo 55:59
    [Google Scholar]
  11. Luria S. E. 1947; Reactivation of irradiated bacteriophage by transfer of self-reproducing units. Proc. Natn. Acad. Sci., U.S.A 33:253
    [Google Scholar]
  12. Luria S. E., Delbrück M. 1942; Interference between inactivated bacterial virus and active virus of the same strain and of a different strain. Archs. Biochem 1:207
    [Google Scholar]
  13. Luria S. E., Human Mary L. 1950; Chromatin staining of bacteria during bacteriophage infection. J. Bact 59:551
    [Google Scholar]
  14. Osman M. A. M. 1965; Pyocine typing of Pseudomonas aeruginosa. J. Clin. Path 18:200
    [Google Scholar]
  15. Paterson Ann C. 1965; Bacteriocinogeny and lysogeny in the genus Pseudomonas. J. Gen. Microbiol 39:295
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-5-3-305
Loading
/content/journal/jmm/10.1099/00222615-5-3-305
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error