1887

Abstract

Summary

The surface growth of several species of clostridia was assessed in terms of the contribution made to the total viable count by spores and vegetative cells respectively. In some cases a large proportion of the surface count was derived from vegetative cells. This conclusion is based on the finding that in many cases the viable count considerably exceeded the maximum estimates for the number of spores in the inocula. Thus vegetative cells may be the significantly viable particles in the surface growth of common pathogenic clostridia. In general, good recovery of the anaerobes tested was obtained with traditional methods.

The possibility that the growth of clinically important anaerobes might be enhanced by the supplementation of 10 per cent, equine blood agar with either (i) sterile iron filings sprinkled on to seeded plates, or (ii) a development of Moore’s cysteine-dithiothreitol system, was explored. There was no evidence that enhancement occurred, except with organisms of the group.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-5-2-211
1972-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/5/2/medmicro-5-2-211.html?itemId=/content/journal/jmm/10.1099/00222615-5-2-211&mimeType=html&fmt=ahah

References

  1. Collee J. G., Rutter J. M., Watt B. 1971; The significantly viable particle: a study of the subculture of an exacting sporing anaerobe. J. Med. Microbiol 4:271
    [Google Scholar]
  2. Cruickshank R. 1968 Medical microbiology. , 11th ed.. (revised reprint) Edinburgh and London:
    [Google Scholar]
  3. Drasar B. S., Crowther J. S. 1971 The cultivation of human intestinal bacteria. In Isolation of anaerobes (Soc. Appl. Bact. Tech. Series no. 5). edited by Shapton D. A., and Board R. G. London and New York: p. 93
    [Google Scholar]
  4. Füzi M., Csukas Zsuzsanna. 1969; New selective medium for the isolation of Clostridium perfringens. Acta microbiol. hung 16:273
    [Google Scholar]
  5. Garcia M. M., McKay K. A. 1969; On the growth and survival of Clostridium septicum in soil. J. Appl. Bact 32:362
    [Google Scholar]
  6. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bact. Rev 14:1
    [Google Scholar]
  7. Lowbury E. J. L., Lilly H. A. 1955; A selective plate medium for Cl. welchii. J. Path Bact 70:105
    [Google Scholar]
  8. Moore W. B. 1968; Solidified media suitable for the cultivation of Clostridium novyi type B. J. Gen. Microbiol. 53:415
    [Google Scholar]
  9. Smith J. W. C., MacIver A. G. 1969; Studies in experimental tetanus infection. J. Med. Microbiol 2:385
    [Google Scholar]
  10. Williams Kathleen, Willis A. T. 1970; A method of performing surface viable counts with Clostridium tetani. J. Med. Microbiol 3:639
    [Google Scholar]
  11. Willis A. T. 1969 Clostridia of wound infection. London: p. 20
    [Google Scholar]
  12. Willis A. T., Hobbs G. 1959; Some new media for the isolation and identification of Clostridia. J. Path. Bact 77:511
    [Google Scholar]
  13. Willis A. T., Williams Kathleen. 1970; Some cultural reactions of Clostridium tetani. J. Med. Microbiol 3:291
    [Google Scholar]
  14. Wilson G. S., Miles A. A. 1964 Topley and Wilson’s Principles of bacteriology and immunity. , 5th ed.. London: p. 2129
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-5-2-211
Loading
/content/journal/jmm/10.1099/00222615-5-2-211
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error