1887

Abstract

Summary

A selective medium for bovis has been developed containing polymyxin B 200 units per ml, carbenicillin 100 μ per ml, amphotericin B 10 μ per ml and trimethoprim 10 or 20 μ per ml in 7H10 oleic acid albumin agar. When the centrifuged deposit from sputum, liquefied by treatment with dithiothreitol, was plated directly on to the selective medium, less than 1 per cent. of plates were contaminated and the viable counts were about 240 per cent. higher than parallel counts after treatment of the deposit with NaOH and plating on 7H10 medium without drugs. A comparison of results on medium with and without the selective drugs showed that the drugs slowed the early growth of in slide culture and reduced viable counts by, at most, 10 per cent. Improved anti-fungal cover could be obtained with no reduction in viable counts by increasing the concentration of amphotericin B to 50 μ per ml.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-5-2-165
1972-05-01
2021-11-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/5/2/medmicro-5-2-165.html?itemId=/content/journal/jmm/10.1099/00222615-5-2-165&mimeType=html&fmt=ahah

References

  1. Cochran W. G. 1957; Analysis of covariance: its nature and uses. Biometrics 13:261
    [Google Scholar]
  2. Cohn M. L., Middlebrook G. 1963; Carbon dioxide-impermeable plastic bags for artificial cultivation of tubercle bacilli. Amer. Rev. Resp. Dis 87:292
    [Google Scholar]
  3. Dickenson Jean M., Ellard G. A., Mitchison D. A. 1968 Suitability of isoniazid and ethambutol for intermittent administration in the treatment of tuberculosis. Tubercle, Lond.: 49351
    [Google Scholar]
  4. Finegold S. M., Sweeney E. E. 1961; New selective and differential medium for coagulase-positive staphylococci allowing rapid growth and strain differentiation. J. Bact 81:636
    [Google Scholar]
  5. Garrod L. P., O’Grady F. 1971 Antibiotic and chemotherapy. , 3rd ed.. Edinburgh
    [Google Scholar]
  6. Gruft H. 1965; Nalidixic acid as a decontaminant in Lo wens tein-Jensen medium. J. Bact 90:829
    [Google Scholar]
  7. Jeffries L. 1959; Novobiocin-tetrathionate broth: a medium of improved selectivity for the isolation of salmonellae from faeces. J. Clin. Path 12:568
    [Google Scholar]
  8. Jones Lois M., Morgan W. J. B. 1958; A preliminary report on a selective medium for the culture of Brucella, including fastidious types. Bull. Wld Hlth Org 19:200
    [Google Scholar]
  9. Lowbury E. J. L., Kidson A., Lilly H. A. 1964; A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci. J. Clin. Path 17:231
    [Google Scholar]
  10. Lowbury E. J. L., Lilly H. A. 1955; A selective plate medium for Cl. welchii. J. Path. Bact 70:105
    [Google Scholar]
  11. Middlebrook G., Cohn M. L. 1958; Bacteriology of tuberculosis; laboratory methods. Amer. J. Publ. Hlth 48:844
    [Google Scholar]
  12. Rieber M., Imaeda T., Cesari I. M. 1969; Bacitracin action on membranes of mycobacteria. J. Gen. Microbiol 55:155
    [Google Scholar]
  13. Selkon J. B., Mitchison D. A. 1957; Viable counting of Mycobacterium tuberculosis in a silica gel medium. J. Gen. Microbiol 16:229
    [Google Scholar]
  14. Stretch J. H., Dickinson Jean M. 1958; Slide culture estimation of sensitivity of Mycobacterium tuberculosis. Ir. J. Med. Sci no. 389:237
    [Google Scholar]
  15. Thayer J. D., Martin J. E. Jr 1964; A selective medium for the cultivation of N. gonorrhoeae and N. meningitidis. Pub. Hlth Rep., Wash 79:49
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-5-2-165
Loading
/content/journal/jmm/10.1099/00222615-5-2-165
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error