1887

Abstract

Summary

The theory that culture products of type-12 group A β-haemolytic streptococci may enzymically render IgG autoimmunogenic was investigated with reference to the production of an autologous immune-complex disease in rabbits.

Six groups of 15 animals were used. Autologous serum that had been incubated with a culture of the test streptococcus was centrifuged and freed from bacteria by filtration and then reintroduced into the donor animals in group I. Purified autologous IgG was similarly treated with streptococcal culture and the bacteriafree material was reintroduced into the donor animals in group II. Control animals in groups III and IV were treated similarly to those in groups I and II, except that streptococci were not included in the incubation step. Animals in groups V and VI received untreated autologous serum and untreated autologous IgG respectively.

Fifty per cent, of the animals in groups I and II developed haematuria and proteinuria, and showed glomerular lesions with deposition of host IgG and β C on the basement-membranes; no streptococcal antigens could be detected in the kidney. Cultures of the injected preparations yielded no streptococci and results of blood culture and antistreptolysin O determinations were negative for all of the animals. The control animals in groups III-VI showed no abnormalities.

The results of this study support the hypothesis that streptococcal modification of IgG may be related to the development of immune-deposit disease.

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1972-02-01
2024-05-20
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References

  1. Barnett E. V., Bluestone R., 3rd Cracchiolo A., Goldberg L. S., Kantor G. L., McIntosh R. M. 1970; Cryoglobulinemia and disease. Ann. Intern. Med. 73:95
    [Google Scholar]
  2. Becker C. G., Murphy G. E. 1968; The experimental induction of glomerulonephritis like that in man by infection with Group A streptococci. J. Exp. Med. 127:1
    [Google Scholar]
  3. Coons A. H. 1956 Histochemistry with labeled antibody. In International review of cytology. ed. by Bourne G. H., Danielli J. F. New York: vol 5 pp 1–23
    [Google Scholar]
  4. Dixon F. J., Feldman J. D., Vazquez J. J. 1961; Experimental glomerulonephritis. The pathogenesis of a laboratory model resembling the spectrum of human glomerulonephritis. J. Exp. Med. 113:899
    [Google Scholar]
  5. Edgington T. S., Glassock R. J., Dixon F. J. 1967; Autologous immune complex pathogenesis of experimental allergic glomerulonephritis. Science, N. Y. 155:1432
    [Google Scholar]
  6. Flodin P., Killander J. 1962; Fractionation of human-serum proteins by gel filtration. Biochim. biophys. Acta 63:403
    [Google Scholar]
  7. Fox E. N. 1964; Antigenicity of the M proteins of group A hemolytic streptococci. J. Immun. 93:826
    [Google Scholar]
  8. Horn H. 1937; The experimental nephropathies. Archs Path. 23:71
    [Google Scholar]
  9. Kobernick S. D. 1952; Experimental rheumatic carditis, periarteritis nodosa and glomerulonephritis. Amer. J. Med. Sci. 224:329
    [Google Scholar]
  10. Lawrence H. S. 1959; Homograft sensitivity: an expression of the immunologic origins and consequences of individuality. Physiol. Rev. 39:811
    [Google Scholar]
  11. Lindberg Lois H., Vosti K. L., Raffel S. 1967; Experimental streptococcal glomerulonephritis in rats. J. Immun. 98:1231
    [Google Scholar]
  12. McIntosh R. M., Kaufman D. B., Kulvinskas C., Grossman B. J. 1970; Cryoglobulins I. Studies on the nature, incidence and clinical significance of serum cryo-proteins in glomerulonephritis. J. Lab. Clin. Med. 75:566
    [Google Scholar]
  13. McIntosh R. M., Kulvinskas C., Kaufman D. B. 1971; Alteration of the chemical composition of human immunoglobulin G by. Streptococcus pyogenes. J. Med. Microbiol. 4:535
    [Google Scholar]
  14. MacNider W. deB. 1924; A review of acute experimental nephritis. Physiol. Rev. 4:595
    [Google Scholar]
  15. Markowitz A. S., Armstrong S. H., Kushner D. S. 1960; Immunological relationships between the rat glomerulus and nephritogenic streptococci. Nature, Lond. 187:1095
    [Google Scholar]
  16. Michael A. F. Jr, Drummond K. N., Good R. A., Vernier R. L. 1966; Acute poststreptococcal glomerulonephritis. Immune deposit disease. J. Clin. Invest. 45:237
    [Google Scholar]
  17. Michael A. F. Jr, Drummond K. N., Vernier R. L., Good R. A. 1964; Immunologic basis of renal disease. Pediat. Clins N. Amer. 11:685
    [Google Scholar]
  18. Ouchterlony Ö. 1958; Diffusion-in-gel methods for immunological analysis. Prog. Allergy 5:1
    [Google Scholar]
  19. Rammelkamp C. H. 1957; Microbiologic aspects of glomerulonephritis. J. Chron. Dis. 5:28
    [Google Scholar]
  20. Rapaport F. T., Markowitz A. S., McCluskey R. T., Hanaoka T., Shimada T. 1969; Induction of renal disease with antisera to Group A streptococcal membranes. Transplantn Proc. 1:981
    [Google Scholar]
  21. Scheidegger J. J. 1955; Une micro-méthode de l’immuno-électrophorése. Int. Archs Allergy Appl. Immun. 7:103
    [Google Scholar]
  22. Tan E. M. 1964; Experimental renal disease induced by streptococci. In The streptococcus, rheumatic fever and glomerulonephritis. ed. by Uhr J. W. Baltimore: p 238
    [Google Scholar]
  23. Tomasi T., Kunkel H. G. 1964 Isolation of 7S and 19S gamma globulins In Methods in medical research. ed. by Eisen H. N. Chicago: vol 10: pp 1–23
    [Google Scholar]
  24. Treser G., Semar M., Ty Antonia, Sagel I., Franklin M. A., Lange K. 1970; Partial characterization of antigenic streptococcal plasma membrane components in acute glomerulonephritis. J. Clin. Invest. 49:762
    [Google Scholar]
  25. Vernier R. L. 1969 Glomerulonephritis. In Textbook of immunopathology. ed. by Miescher P. A., Müer-Eberhard H. New York: p 376
    [Google Scholar]
  26. Zinneman H. H., Levi D., Seal U. S. 1968; On the nature of cryoglobulins. J. Immun. 100:594
    [Google Scholar]
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