%0 Journal Article %A Matsumoto, Tetsuya %A Furuya, Nobuhiko %A Tateda, Kazuhiro %A Miyazaki, Shuichi %A Ohno, Akira %A Ishii, Yoshikazu %A Hirakata, Yoichi %A Yamaguchi, Keizo %T Effect of Passive Immunotherapy on Murine Gut-Derived Sepsis Caused by Pseudomonas Aeruginosa %D 1999 %J Journal of Medical Microbiology, %V 48 %N 8 %P 765-770 %@ 1473-5644 %R https://doi.org/10.1099/00222615-48-8-765 %I Microbiology Society, %X Summary The effect of passive immunotherapy with antisera against heat-killed Pseudomonas aeruginosa and three of its exo-enzymes (elastase, alkaline protease and exotoxin A) in gut-derived P. aeruginosa sepsis was evaluated. Mice were given a suspension of P. aeruginosa strain D4 in their drinking water, together with ampicillin (200 mg/kg) to disrupt the normal bacterial flora. Cyclophosphamide was then administered to induce translocation of P. aeruginosa that had colonised the gastrointestinal tract so that gut-derived septicaemia was produced. In this model, intraperitoneal administration of antiserum against heat-killed bacteria, 100 μl/mouse, twice a day for 3 consecutive days significantly increased the survival rate over that of mice treated with normal rabbit serum. Antiserum against elastase, alkaline protease, or a combination of these two antisera, failed to provide significant protection. In contrast, antiserum against exotoxin A significantly increased the survival rate over that of mice treated with normal rabbit serum. These results indicate that passive immunisation with antiserum against heat-killed bacteria and exotoxin A, but not with antiserum against either elastase or alkaline protease, protects mice against gut-derived sepsis caused by P. aeruginosa. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-48-8-765