@article{mbs:/content/journal/jmm/10.1099/00222615-48-8-757, author = "Sharma, Anil K. and Verma, Indu and Tewari, R. and Khuller, G. K.", title = "Adjuvant Modulation of T-cell Reactivity to 30-kDa Secretory Protein of Mycobacterium Tuberculosis H37Rv and its Protective Efficacy Against Experimental Tuberculosis", journal= "Journal of Medical Microbiology", year = "1999", volume = "48", number = "8", pages = "757-763", doi = "https://doi.org/10.1099/00222615-48-8-757", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-48-8-757", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary The immunoprotective behaviour of the 30-kDa secretory glycoprotein of Mycobacterium tuberculosis H37Rv has been investigated in different adjuvant systems in two mouse strains, NMR1 and C57BL/6J. In comparison with Freund's incomplete adjuvant (FIA) and dimethyldioctadecyl ammonium bromide (DDA), the 30-kDa glycoprotein complexed with polylactide-co-glycolide microparticles (PLG-MPs) induced maximum immuno-reactivity in the two mouse strains. As compared with controls, immunisation with 30-kDa-PLG-MPs resulted in significantly greater protection in animals challenged with 1 × LD50 of M. tuberculosis H37Rv on the basis of survival rates and number of cfu in the infected organs 30 days after challenge. The degree of protection provided by 30-kDa-PLG-MPs was similar to that obtained with 30-kDa-FIA and higher than BCG immunisation. These findings suggest that biodegradable PLG microparticles can be used as an efficient carrier system for the key immunoprotective 30-kDa secretory protein antigen of M. tuberculosis H37Rv.", }