PCR and restriction fragment length polymorphism (RFLP) typing of flagellin genes (C) from 57 clinical isolates of indicated that only type II flagellins were present. Twenty-two isolates previously identified as the epidemic UK cystic fibrosis strain were indistinguishable by this method, as were 11 isolates from a pseudo-outbreak in Senegal. Other clinical isolates, including 19 from disparate sources in Malaysia, were separated into nine C RFLP groups, exhibiting a large degree of divergence. When isolates were indistinguishable by C genotyping, their similarity was confirmed by whole genome macro-restriction analysis with pulsed-field gel electrophoresis following I digestion. The variation in C sequences of was far greater than that with , supporting the view that ‘’ as currently defined, may comprise several different genomic species.


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